RT Journal Article
T1 The formin INF2 regulates basolateral-to-apical transcytosis and lumen formation in association with Cdc42 and MAL2
A1 Madrid González, Ricardo
A1 Aranda Gómez, Juan Francisco
A1 Rodríguez Fraticelli, Alejo
A1 Ventimiglia, Leandro
A1 Andrés Delgado, Laura
A1 Shehata, Mona
A1 Fanayan, Susan
A1 Shahheydari, Hamideh
A1 Gómez, Sergio
A1 Jiménez, Alberto
A1 Martín Belmonte, Fernando
A1 Byrne, Jennifer Anne
A1 Alonso, Miguel
AB Transcytosis is a widespread pathway for apical targeting in epithelial cells. MAL2, an essential protein of the machinery for apical transcytosis, functions by shuttling in vesicular carriers between the apical zone and the cell periphery. We have identified INF2, an atypical formin with actin polymerization and depolymerization activities, which is a binding partner of MAL2. MAL2-positive vesicular carriers associate with short actin filaments during transcytosis in a process requiring INF2. INF2 binds Cdc42 in a GTP-loaded-dependent manner. Cdc42 and INF2 regulate MAL2 dynamics and are necessary for apical transcytosis and the formation of lateral lumens in hepatoma HepG2 cells. INF2 and MAL2 are also essential for the formation of the central lumen in organotypic cultures of epithelial MDCK cells. Our results reveal a functional mechanism whereby Cdc42, INF2, and MAL2 are sequentially ordered in a pathway dedicated to the regulation of transcytosis and lumen formation.
PB Elsevier
SN 1534-5807
YR 2010
FD 2010
LK https://hdl.handle.net/20.500.14352/99128
UL https://hdl.handle.net/20.500.14352/99128
LA eng
NO Madrid, Ricardo, et al. «The Formin INF2 Regulates Basolateral-to-Apical Transcytosis and Lumen Formation in Association with Cdc42 and MAL2». Developmental Cell, vol. 18, n.o 5, mayo de 2010, pp. 814-27. https://doi.org/10.1016/j.devcel.2010.04.001.
NO This work was supported by grants (BFU2006-01925, BFU2009-07886, and CONSOLIDER COAT CSD2009-00016) to M.A.A. from the Ministerio de Ciencia e Innovación (MICINN), Spain. R.M. is the holder of a contract from the Ramón y Cajal Program of the MICINN.
NO Ministerio de Ciencia e Innovación (España)
DS Docta Complutense
RD 6 abr 2025