RT Book, Section
T1 Differential Scanning Calorimetry of Protein–Lipid Interactions
A1 Cañadas Benito, Olga
A1 Casals Carro, María Cristina
A2 Kleinschmidt, Jörg H.
AB Differential scanning calorimetry (DSC) is a highly sensitive nonperturbing technique used for studying the thermodynamic properties of thermally induced transitions. Since these properties might be affected by ligand binding, DSC is particularly useful for the characterization of protein interactions with biomimetic membranes. The advantages of this technique over other methods consist in the direct measurement of intrinsic thermal properties of the samples, requiring no chemical modifications or extrinsic probes. This chapter describes the basic theory of DSC and provides the reader with an understanding of the capabilities of DSC instrumentation and the type of information that can be achieved from DSC studies of lipid-protein interactions. In particular, the chapter provides a detailed analysis of DSC data to assess the effects of proteins on biomimetic membranes.
PB Humana Press
SN 9781493995127
YR 2019
FD 2019
LK https://hdl.handle.net/20.500.14352/14041
UL https://hdl.handle.net/20.500.14352/14041
LA eng
NO Ministerio de Economía y Competitividad (MINECO)
NO Instituto de Salud Carlos III (ISCIII)
DS Docta Complutense
RD 12 abr 2025