RT Journal Article
T1 Microscopic Viscosity of Neuronal Plasma Membranes Measured Using Fluorescent Molecular Rotors: Effects of Oxidative Stress and Neuroprotection
A1 Kubánková, Markéta
A1 Summers, Peter A.
A1 López Duarte, Ismael
A1 Kiryushko, Darya
A1 Kuimova, Marina K.
AB Molecular mobility in neuronal plasma membranes is a crucial factor in brain function. Microscopic viscosity is an important parameter that determines molecular mobility. This study presents the first direct measurement of the microviscosity of plasma membranes of live neurons. Microviscosity maps were obtained using fluorescence lifetime imaging of environment-sensing dyes termed “molecular rotors”. Neurons were investigated both in the basal state and following common neurodegenerative stimuli, excitotoxicity, or oxidative stress. Both types of neurotoxic challenges induced microviscosity decrease in cultured neurons, andoxidant-induced membrane fluidification was counteracted by the wide-spectrum neuroprotectant, the H3 peptide. Theseresults provide new insights into molecular mobility in neuronal membranes, paramount for basic brain function, and suggestthat preservation of membrane stability may be an important aspect of neuroprotection in brain insults and neurodegenerativedisorders.
PB American Chemical Society (ACS)
SN 1944-8244
SN 1944-8252
YR 2019
FD 2019-09-12
LK https://hdl.handle.net/20.500.14352/115748
UL https://hdl.handle.net/20.500.14352/115748
LA eng
NO Kubánková M, Summers PA, López-Duarte I, Kiryushko D, Kuimova MK. Microscopic viscosity of neuronal plasma membranes measured using fluorescent molecular rotors: effects of oxidative stress and neuroprotection. ACS Appl Mater Interfaces [Internet]. 9 de octubre de 2019 [citado 23 de enero de 2025];11(40):36307-15. Disponible en: https://pubs.acs.org/doi/10.1021/acsami.9b10426
NO Michael J. Fox Parkinson’s Research Foundation
NO Engineering & Physical Sciences Research Council
DS Docta Complutense
RD 20 ene 2026