RT Journal Article
T1 Ovine Mannheimia haemolytica isolates from lungs with and without pneumonic lesions belong to similar genotypes
A1 García Álvarez, Andrés
A1 Fernández-Garayzábal Fernández, José Francisco
A1 Chaves, Fernando
A1 Pinto, Chris
A1 Cid Vázquez, María Dolores
AB This study investigated the genetic characteristics of 121 ovine Mannheimia haemolytica isolates from lungs with (n = 75) and without pneumonic lesions (n = 46) using multilocus sequence typing (MLST), virulence-associated gene typing and pulsed-field gel electrophoresis (PFGE). Twelve STs were identified with most isolates (81%) belonged to ST16, ST28 and ST8. Analysis of the M. haemolytica MLST Database indicate a wide distribution of these genotypes in small ruminants, never reported in bovine isolates. This could suggest the adaptation of certain genetic lineages of M. haemolytica to small ruminants. e-BURST analysis grouped most STs into three clonal complexes (CC2, CC8 and CC28), consistent with a clonal population structure of M. haemolytica. Virulence-associated gene typing identified five virulence profiles in 64% and 65.1% of the M. haemolytica isolates from lungs with and without pneumonic lesions, respectively. These data suggest that M. haemolytica isolates from the lungs with and without pneumonic lesions are genetically homogeneous. By PGFE analysis a high level of genetic diversity was observed not only within isolates from lungs without pneumonic lesions but also among isolates from pneumonic lesions (GD 0.69 and GD 0.66, respectively; P > 0.05). These results indicate that multiple strains of M. haemolytica may be associated with individual cases of pneumonia in sheep.
PB Elsevier
SN 0378-1135
YR 2018
FD 2018
LK https://hdl.handle.net/20.500.14352/93967
UL https://hdl.handle.net/20.500.14352/93967
LA eng
NO Garcia-Alvarez A, Fernandez-GarayzabalbJF, Chaves F, Pinto C and Cid D. Ovine Mannheimia haemolytica isolates from lungs with and without pneumonic lesions belong to similar genotypes. Veterinary Microbiology, 219:80-86. 2018. (A). ISSN: 0378-1135. Impact factor: 2.791. Category: Veterinary Sciences, Quartile: 1, Position: 7 of 141. DOI: 10.1016/j.vetmic.2018.04.012
NO 2018 Elsevier B.V. All rights reserved.Autor de correspondencia: Mª Dolores Cid Vázquez;C. Pinto was supported by the Programme Alban, the European Union Programme of High Level Scholarships for Latin America, scholarship No. E07D404011PE.
NO Ministerio de Ciencia, Innovación y Universidades (España)
NO Comunidad de Madrid
NO European Commission
DS Docta Complutense
RD 28 abr 2025