RT Journal Article
T1 Rapid micromotor-based naked-eye immunoassay
A1 Esteban Fernandez de Avila, Berta
A1 Zhao, Mingjiao
A1 Campuzano, Susana
A1 Ricci, Francesco
A1 Pingarrón Carrazón, José Manuel
A1 Mascini, Marcelo
A1 Wang, Joseph
AB A dynamic micromotor-based immunoassay, exemplified by cortisol detection, based on the use of tubular micromotors functionalized with a specific antibody is described. The use of antibody-functionalized micromotors offers huge acceleration of both direct and competitive cortisol immunoassays, along with greatly enhanced sensitivity of direct and competitive immunoassays. The dramatically improved speed and sensitivity reflect the greatly increased likelihood of antibody-cortisol contacts and fluid mixing associated with the dynamic movement of these microtube motors and corresponding bubble generation that lead to a highly efficient and rapid recognition process. Rapid naked-eye detection of cortisol in the sample is achieved in connection to use of horseradish peroxidase (HRP) tag and TMB/H2O2 system. Key parameters of the competitive immunoassay (e.g., incubation time and reaction volume) were optimized. This fast visual micromotor-based sensing approach enables “on the move” specific detection of the target cortisol down to 0.1 μg mL−1 in just 2 min, using ultrasmall (50 µL) sample volumes.
PB Elsevier
SN 0039-9140
YR 2017
FD 2017-05-15
LK https://hdl.handle.net/20.500.14352/18814
UL https://hdl.handle.net/20.500.14352/18814
LA eng
NO Unión Europea. H2020
NO Defense Threat Reduction Agency Joint Science and Technology Office for Chemical and Biological Defense
DS Docta Complutense
RD 20 ago 2024