RT Journal Article
T1 A useful tool for the safe diagnosis and control of the two main pandemics of the XXI century: COVID-19 and African Swine Fever disease
A1 Barroso Arévalo, Sandra
A1 Díaz Frutos, Marta
A1 Kosowska, Aleksandra
A1 Pérez Sancho, Marta
A1 Domínguez Rodríguez, Lucas José
A1 Sánchez-Vizcaíno Rodríguez, José Manuel
A2 Douglas Gladue, 
AB The COVID-19 pandemic and the disease triggered by the African Swine Fever virus are currently two of the main problems regarding public and animal health, respectively. Although vaccination seems to be the ideal tool for controlling these diseases, it has several limitations. Therefore, early detection of the pathogen is critical in order to apply preventive and control measures. Real-time PCR is the main technique used for the detection of both viruses, which requires previous processing of the infectious material. If the potentially infected sample is inactivated at the time of sampling, the diagnosis will be accelerated, impacting positively on the diagnosis and control of the disease. Here, we evaluated the inactivation and preservation properties of a new surfactant liquid for non-invasive and environmental sampling of both viruses. Our results demonstrated that the surfactant liquid effectively inactivates SARS-CoV-2 and African Swine Fever virus in only five minutes, and allows for the preservation of the genetic material for long periods even at high temperatures such as 37°C. Hence, this methodology is a safe and useful tool for recovering SARS-CoV-2 and African Swine Fever virus RNA/DNA from different surfaces and skins, which has significant applied relevance in the surveillance of both diseases.
SN 1932-6203
YR 2023
FD 2023-03-06
LK https://hdl.handle.net/20.500.14352/107552
UL https://hdl.handle.net/20.500.14352/107552
LA eng
NO Barroso-Arévalo S, Díaz-Frutos M, Kosowska A, Pérez-Sancho M, Domínguez L, Sánchez-Vizcaíno JM (2023) A useful tool for the safe diagnosis and control of the two main pandemics of the XXI century: COVID-19 and African Swine Fever disease. PLoS ONE 18(3): e0282632. https://doi.org/10.1371/journal.pone.0282632
NO About the AuthorsSandra Barroso-Arévalo. ROLES Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Writing – original draft, Writing – review & editingMarta Díaz-Frutos. ROLES Conceptualization, Investigation, Methodology, Writing – review & editingAleksandra Kosowska. ROLES Conceptualization, Investigation, Methodology, Writing – review & editingMarta Pérez-Sancho. ROLES Conceptualization, MethodologyLucas Domínguez. ROLES Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Validation, Writing – review & editingJosé Manuel Sánchez-Vizcaíno. ROLES Conceptualization, Funding acquisition, Investigation, Writing – review & editing
NO European Union
NO Comunidad de Madrid
DS Docta Complutense
RD 2 may 2025