RT Journal Article
T1 New trends for a classical enzyme: Papain, a biotechnological success story in the food industry
A1 Fernández Lucas, Jesús
A1 Castañeda, Daniel
A1 Hormigo, Domingo
AB Background. In recent years, proteases have arisen as standard biocatalysts in many industrial processes in different fields, such as pharmaceutical, medicine, detergent manufacturing and food science. Among them, papain is undoubtedly one of the most frequently studied and widely used proteases in the food industry around the world. However, the latest advances in recombinant papain expression systems, genetically engineered biocatalysts, new purification and isolation strategies, and enzymatic immobilization will enhance the development of new applications of papain, as well as improve and optimize classical applications. Scope and approach. This review addresses not only the latest advances in classic applications, such as meat tenderization and protein hydrolysates, but also the most innovative applications in different industries such as food, animal feed, bioactive peptides production, water treatment, baking and brewing, among many others. In addition, papain is a perfect example of a successful industrial enzyme that covers all the steps of the biocatalytic cycle that are necessary for the industrial implementation of any biocatalyst. This cycle includes the production and extraction of the enzyme concerned (from natural or recombinant sources), functional and structural characterization, genetic improvement, immobilization and, finally, industrial application. This review describes the complete biocatalytic cycle of papain.Key findings and conclusions. Papain is clearly a case of industrial and commercial success over the last 40 years. The key to this success has been continual biotechnological and process engineering innovation, which has opened up a new range of possibilities for this exciting biocatalyst. However, further efforts are needed in protein engineering and characterization of new mutants to reach the full potential of this enzyme.
PB Elsevier
SN 0924-2244
YR 2017
FD 2017
LK https://hdl.handle.net/20.500.14352/110444
UL https://hdl.handle.net/20.500.14352/110444
LA eng
NO Fernández-Lucas, J., Castañeda, D., & Hormigo, D. (2017). New trends for a classical enzyme: Papain, a biotechnological success story in the food industry [Review of New trends for a classical enzyme: Papain, a biotechnological success story in the food industry]. Trends in Food Science and Technology, 68, 91-101. Elsevier Ltd. https://doi.org/10.1016/J.TIFS.2017.08.017
NO Fundación Santander
DS Docta Complutense
RD 6 abr 2025