RT Journal Article
T1 Isolation and characterization of extracellular vesicles from natural killer cells
A1 Dosil, Sara G.
A1 Sánchez Madrid, Francisco
A1 Fernández Messina, Lola María
AB Natural killer (NK) cells are critical components of the immune system, recognized for their ability to identify and eliminate target cells undergoing various forms of stress. Beyond their direct cytotoxic activity, NK cells secrete extracellular vesicles (EVs), which serve as mediators of intercellular communication. These vesicles play an essential role in modulating immune responses and influencing tumoral and post-infection microenvironments. In this study, we present a detailed methodology for the isolation and expansion of human primary NK cells, the accumulation of their EVs, and the subsequent procedures for their isolation and characterization.
PB Wiley
SN 2691-1299
YR 2025
FD 2025-04
LK https://hdl.handle.net/20.500.14352/120933
UL https://hdl.handle.net/20.500.14352/120933
LA eng
NO Dosil SG, Sánchez‐Madrid F, Fernández‐Messina L. Isolation and Characterization of Extracellular Vesicles from Natural Killer Cells. Current Protocols 2025;5:e70125. https://doi.org/10.1002/cpz1.70125.
NO Funding:This study was funded by the AECC (grant no. PRYCO223003PEIN), the Spanish Ministry of Science and Innovation (grant no. PID2023\u2010149541OB\u2010I00), and La Caixa Banking Foundation (grant no. HR23\u201000837).
NO Ministerio de Ciencia e Innovación (España)
NO Asociación Española Contra el Cáncer
NO Fundación Bancaria ”la Caixa”
DS Docta Complutense
RD 23 dic 2025