RT Journal Article T1 Combined use of Schizosaccharomyces pombe and a Lachancea thermotolerans strain with a high malic acid consumption ability for wine production A1 Vicente, Javier A1 Kelanne, Niina A1 Navascués, Eva A1 Calderón, Fernando A1 Santos de la Sen, Antonio A1 Marquina Díaz, Domingo A1 Yang, Baoru A1 Benito, Santiago AB The development of new fermentative strategies exploiting the potential of different wine-related species is of great interest for new winemaking conditions and consumer preferences. One of the most promising non-conventional approaches to wine fermentation is the combined use of deacidifying and acidifying yeasts. Lachancea thermotolerans shows several other properties besides lactic acid production; among them, high malic acid consumption is of great interest in the production of red wines for avoiding undesirable refermentations once bottled. The combination of a L. thermotolerans strain that is able to consume malic acid with a Schizosaccharomyces pombe strain helps to ensure malic acid elimination during alcoholic fermentation while increasing the final acidity by lactic acid production. To properly assess the influence of this alternative strategy, we developed combined fermentations between specific strains of L. thermotolerans and S. pombe under sequential inoculation. Both species showed a great performance under the studied conditions, influencing not only the acidity but also the aromatic compound profiles of the resulting wines. The new proposed biotechnological strategy reduced the final concentrations of ethanol, malic acid and succinic acid, while it increased the concentrations of lactic acid and esters. PB MDPI SN 2311-5637 YR 2023 FD 2023-02-11 LK https://hdl.handle.net/20.500.14352/87586 UL https://hdl.handle.net/20.500.14352/87586 LA eng NO Ministerio de Ciencia e Innovación (MICIN) NO Centro para el Desarrollo Tecnológico Industrial (CDTI) DS Docta Complutense RD 6 abr 2025