RT Journal Article
T1 Production of multiple bacteriocins, including the novel bacteriocin gassericin M, by Lactobacillus gasseri LM19, a strain isolated from human milk
A1 Garcia-Gutierrez, Enriqueta
A1 O'Connor, Paula M
A1 Colquhoun, Ian J
A1 Vior, Natalia M
A1 Rodríguez Gómez, Juan Miguel
A1 Mayer, Melinda J
A1 Cotter, Paul D
A1 Narbad, Arjan
AB Bacteriocins are antimicrobial peptides produced by bacteria, and their production is regarded as a desirable probiotic trait. We found that Lactobacillus gasseri LM19, a strain isolated from human milk, produces several bacteriocins, including a novel bacteriocin, gassericin M. These bacteriocins were purified from culture and synthesised to investigate their activity and potential synergy. L. gasseri LM19 was tested in a complex environment mimicking human colon conditions; it not only survived, but expressed the seven bacteriocin genes and produced short-chain fatty acids. Metagenomic analysis of these in vitro colon cultures showed that co-inoculation of L. gasseri LM19 with Clostridium perfringens gave 16S ribosomal RNA metagenomic profiles with more similarity to controls than to vessels inoculated with C. perfringens alone. These results indicate that L. gasseri LM19 could be an interesting candidate for maintaining homeostasis in the gut environment.
PB Springer
SN 0175-7598
YR 2020
FD 2020
LK https://hdl.handle.net/20.500.14352/129364
UL https://hdl.handle.net/20.500.14352/129364
LA eng
NO Garcia-Gutierrez, E., O'Connor, P. M., Colquhoun, I. J., Vior, N. M., Rodríguez, J. M., Mayer, M. J., Cotter, P. D., & Narbad, A. (2020). Production of multiple bacteriocins, including the novel bacteriocin gassericin M, by Lactobacillus gasseri LM19, a strain isolated from human milk. Applied microbiology and biotechnology, 104(9), 3869–3884. https://doi.org/10.1007/s00253-020-10493-3
NO Walsh Fellowship Project
NO BBSRC Institute Strategic Programme
NO APC Microbiome Institute
DS Docta Complutense
RD 31 dic 2025