TY  - JOUR
AU  - Siar, El-Hocine
AU  - Morellon-Sterling, Roberto
AU  - Carballares, Diego
AU  - Rocha Martín, Javier
AU  - Barbosa, Oveimar
AU  - Bolívar Bolívar, Juan Manuel
AU  - Fernandez-Lafuente, Roberto
PY  - 2023
DO  - 10.1016/j.procbio.2023.07.029
SN  - 1359-5113
UR  - https://hdl.handle.net/20.500.14352/101622
T2  - Process Biochemistry
AB  - The immobilization of ficin extract (sulfidryl proteases) on glyoxyl agarose beads stabilized it, but there is a clear stability maximum after 3 h of immobilization, being the 24 h biocatalyst less stable when thermally inactivated in presence of air....
LA  - eng
M2  - 289
PB  - Elsevier
KW  - Enzyme stabilization
KW  - Enzyme immobilization
KW  - Cys oxidation
KW  - Multipoint covalent attachment
KW  - Enzyme distortion
KW  - Enzyme rigidification
TI  - Glyoxyl-ficin: an example where a more intense multipoint covalent attachment may decrease enzyme stability
TY  - journal article
VL  - 132
ER  -