RT Journal Article
T1 Poly (ADP-ribose) polymerase 3 (PARP3), a potential repressor of telomerase activity
A1 Fernández-Marcelo, Tamara
A1 Frías, Cristina
A1 Pascua, Irene
A1 Juan Chocano, María Del Carmen De
A1 Head, Jacqueline
A1 Gómez, Ana
A1 Hernando Trancho, Florentino
A1 Jarabo Sarceda, José Ramón
A1 Díaz-Rubio García, Eduardo
A1 Torres García, Antonio José
A1 Rouleau, Michèle
A1 Benito De Las Heras, Manuel R.
A1 Iniesta, Pilar
A1 Iniesta Serrano, María Pilar
AB BackgroundConsidering previous result in Non-Small Cell Lung Cancer (NSCLC), we investigated in human cancer cells the role of PARP3 in the regulation of telomerase activity.MethodsWe selected A549 (lung adenocarcinoma cell line) and Saos-2 (osteosarcoma cell line), with high and low telomerase activity levels, respectively. The first one was transfected using a plasmid construction containing a PARP3 sequence, whereas the Saos-2 cells were submitted to shRNA transfection to get PARP3 depletion. PARP3 expression on both cell systems was evaluated by real-time quantitative PCR and PARP3 protein levels, by Western-blot. Telomerase activity was determined by TRAP assay.ResultsIn A549 cells, after PARP3 transient transfection, data obtained indicated that twenty-four hours after transfection, up to 100-fold increased gene expression levels were found in the transfected cells with pcDNA/GW-53/PARP3 in comparison to transfected cells with the empty vector. Moreover, 48 hours post-transfection, telomerase activity decreased around 33%, and around 27%, 96 hours post-transfection. Telomerase activity average ratio was 0.67 ± 0.05, and 0.73 ± 0.06, respectively, with significant differences. In Saos-2 cells, after shRNA-mediated PARP3 silencing, a 2.3-fold increase in telomerase activity was detected in relation to the control.ConclusionOur data indicated that, at least in some cancer cells, repression of PARP3 could be responsible for an increased telomerase activity, this fact contributing to telomere maintenance and, therefore, avoiding genome instability.
PB BMC
YR 2014
FD 2014-02-15
LK https://hdl.handle.net/20.500.14352/93262
UL https://hdl.handle.net/20.500.14352/93262
LA eng
NO Fernández-Marcelo T, Frías C, Pascua I, De Juan C, Head J, Gómez A, et al. Poly (ADP-ribose) polymerase 3 (PARP3), a potential repressor of telomerase activity. J Exp Clin Cancer Res 2014;33:19. https://doi.org/10.1186/1756-9966-33-19.
NO Fundación de Investigación Médica Mutua Madrileña
NO Neumomadrid
NO Banco Santander
NO Universidad Complutense de Madrid
NO Red Temática de Investigación Cooperativa en Cáncer
DS Docta Complutense
RD 9 abr 2025