RT Journal Article
T1 Physiological Doses of Oleic and Palmitic Acids Protect Human Endothelial Cells from Oxidative Stress
A1 Palomino Ruiz-Poveda, Olga María
A1 Giordiani, Veronica
A1 Chowen, Julie
A1 Fernández Alfonso, María Soledad
A1 Goya, Luis
AB Oxidative stress has been proposed to be a pathogenic mechanism to induce endothelial dysfunction and the onset of cardiovascular disease. Elevated levels of free fatty acids can cause oxidative stress by increasing mitochondrial uncoupling but, at physiological concentrations, they are essential for cell and tissue function and olive oil free fatty acids have proved to exhibit beneficial effects on risk factors for cardiovascular disease. We hypothesize that realistic concentrations within the physiological range of oleic (OA) and palmitic (PA) acids could be beneficial in the prevention of oxidative stress in vascular endothelium. Hence, pre-treatment and co-treatment with realistic physiological doses of palmitic and oleic acids were tested on cultured endothelial cells submitted to a chemically induced oxidative stress to investigate their potential chemo-protective effect. Cell viability and markers of oxidative status: reactive oxygen species (ROS), reduced glutathione (GSH), malondialdehyde (MDA), glutathione peroxidase (GPx) and glutathione reductase (GR) were evaluated. As a conclusion, the increased ROS generation induced by stress was significantly prevented by a pre- and co-treatment with PA or OA. Moreover, pre- and co-treatment of cells with FFAs recovered the stress-induced MDA concentration to control values and significantly recovered depleted GSH and normalized GPx and GR activities. Finally, pre- and co-treatment of cells with physiological concentrations of PA or OA in the low micromolar range conferred a substantial protection of cell viability against an oxidative insult.
PB MDPI
SN 1420-3049
YR 2022
FD 2022-08-16
LK https://hdl.handle.net/20.500.14352/110235
UL https://hdl.handle.net/20.500.14352/110235
LA eng
NO Palomino, O.M.; Giordani, V.; Chowen, J.; Fernández-Alfonso, M.S.; Goya, L. Physiological Doses of Oleic and Palmitic Acids Protect Human Endothelial Cells from Oxidative Stress. Molecules 2022, 27, 5217. https://doi.org/10.3390/ molecules27165217
NO Ministerio de Economía, Industria y Competitividad (España)
DS Docta Complutense
RD 17 abr 2025