RT Journal Article
T1 Membrane depolarization regulates AMPA receptor subunit expression in cerebellar granule cells in culture
A1 Incontro, Salvatore
A1 Ramírez Franco, José Jorge
A1 Sánchez-Prieto Borja, José
A1 Torres Molina, Magdalena Isabel
AB The physiological responses of AMPA receptors can be modulated through the differential expression of their subunits and by modifying their number at the cell surface. Here we have studied the expression of AMPA receptor subunits (GluR1–4) mRNAs in cerebellar granule cells grown in depolarizing (25 mM K+) medium, and we have evaluated the effect of decreasing the [K+] in the culture medium for 24 h on both GluR1–4 expression (both mRNA and protein) and their presence at the plasma membrane. The expression of the four AMPAR subunits increases as the [K+] decreases, although the increase in GluR2 and GluR3 was only observed in the cell soma but not in the dendrites. Calcium entry through L-type calcium channel and CaMKIV activation are responsible for the reduction in the expression of AMPA receptor subunits in cells cultured in depolarizing conditions. Indeed, prolonged reduction of extracellular [K+] or blockage of L-type calcium channels enhanced both the surface insertion of the four AMPAR subunits and the AMPA response measured through intracellular calcium increase. These findings reveal a balanced increase in functional AMPA receptors at the surface of cells that can trigger strong increases in calcium in response to the persistent reduction of calcium entry.
PB Elsevier
SN 0167-4889
YR 2011
FD 2011
LK https://hdl.handle.net/20.500.14352/129687
UL https://hdl.handle.net/20.500.14352/129687
LA eng
NO Incontro, S., Ramírez-Franco, J., Sánchez-Prieto, J., & Torres, M. (2011). Membrane depolarization regulates AMPA receptor subunit expression in cerebellar granule cells in culture. Biochimica et biophysica acta, 1813(1), 14–26. https://doi.org/10.1016/j.bbamcr.2010.10.016
NO Ministerio de Ciencia e Innovación (España)
NO Ministerio de Sanidad y Consumo (España)
NO Comunidad de Madrid
NO Universidad Complutense de Madrid
DS Docta Complutense
RD 20 ene 2026