RT Journal Article
T1 Functional organization of the HIV lipid envelope
A1 Huarte, Nerea
A1 Carravilla, Pablo
A1 Cruz Rodríguez, Antonio
A1 Lorizate, Maier
A1 Nieto-Garay, Jon A.
A1 Kräusslich, Hans-Georg
A1 Pérez-Gil, Jesús
A1 Requejo Isidro, José
A1 Nieva, José L.
AB The chemical composition of the human immunodeficiency virus type 1 (HIV-1) membrane is critical for fusion and entry into target cells, suggesting that preservation of a functional lipid bilayer organization may be required for efficient infection. HIV-1 acquires its envelope from the host cell plasma membrane at sites enriched in raft-type lipids. Furthermore, infectious particles display aminophospholipids on their surface, indicative of dissipation of the inter-leaflet lipid asymmetry metabolically generated at cellular membranes. By combining two-photon excited Laurdan fluorescence imaging and atomic force microscopy, we have obtained unprecedented insights into the phase state of membranes reconstituted from viral lipids (i.e., extracted from infectious HIV-1 particles), established the role played by the different specimens in the mixtures, and characterized the effects of membrane-active virucidal agents on membrane organization. In determining the molecular basis underlying lipid packing and lateral heterogeneity of the HIV-1 membrane, our results may help develop compounds with antiviral activity acting by perturbing the functional organization of the lipid envelope.
PB Nature Publishing Group
SN 2045-2322, ESSN: 2045-2322
YR 2016
FD 2016
LK https://hdl.handle.net/20.500.14352/23379
UL https://hdl.handle.net/20.500.14352/23379
LA eng
NO Ministerio de Economía y Competitividad (MINECO)
NO Comunidad de Madrid
NO National Institute of Health(USA)
NO Gobierno Vasco
NO Deutsche Forschungsgemeinschaft
DS Docta Complutense
RD 6 may 2024