RT Journal Article
T1 Imaging tumor microscopic viscosity in vivo using molecular rotors
A1 Shimolina, Lyubov’ E.
A1 Izquierdo, Maria Angeles
A1 López Duarte, Ismael
A1 Bull, James A.
A1 Shirmanova, Marina V.
A1 Klapshina, Larisa G.
A1 Zagaynova, Elena V.
A1 Kuimova, Marina K.
AB The microscopic viscosity plays an essential role in cellular biophysics by controlling the rates of diffusion and bimolecular reactions within the cell interior. While several approaches have emerged that have allowed the measurement of viscosity and diffusion on a single cell level in vitro, the in vivo viscosity monitoring has not yet been realized. Here we report the use of fluorescent molecular rotors in combination with Fluorescence Lifetime Imaging Microscopy (FLIM) to image microscopic viscosity in vivo, both on a single cell level and in connecting tissues of subcutaneous tumors in mice. We find that viscosities recorded from single tumor cells in vivo correlate well with the in vitro values from the same cancer cell line. Importantly, our new method allows both imaging and dynamic monitoring of viscosity changes in real time in live animals and thus it is particularly suitable for diagnostics and monitoring of the progress of treatments that might be accompanied by changes in microscopic viscosity.
PB Springer Science and Business Media LLC
SN 2045-2322
YR 2017
FD 2017-01-30
LK https://hdl.handle.net/20.500.14352/114911
UL https://hdl.handle.net/20.500.14352/114911
LA eng
NO Shimolina LE, Izquierdo MA, López-Duarte I, Bull JA, Shirmanova MV, Klapshina LG, et al. Imaging tumor microscopic viscosity in vivo using molecular rotors. Sci Rep [Internet]. 30 de enero de 2017 [citado 17 de enero de 2025];7(1):41097. Disponible en: https://www.nature.com/articles/srep41097
NO Russian Foundation for Basic Research
NO European Commission
DS Docta Complutense
RD 12 abr 2025