RT Journal Article
T1 Electrospun nanofibers for localized drug release of a neuroprotective natural extract of Usnea ghattensis
A1 Fernández Fernández, María De La Cabeza
A1 Sánchez Gómez-Serranillos, Marta
A1 Lozano-Chamizo, Laura
A1 Abreu, Ana Cristina
A1 Anguis-Morillas, Ana
A1 Dulare Devi, Pradeep Divakar
A1 Marciello, Marzia
A1 Filice, Marco
A1 Gonzalez-Rumayor, Victor
A1 Fernandez, Ignacio
A1 Contreras Cáceres, Rafael
A1 Gómez-Serranillos Cuadrado, María Pilar
AB This research is based on the incorporation of the methanolic extract of the Usnea ghattensis into poly (caprolactone) (PCL) nanofibers (NFs) to investigate the capacity in reducing reactive oxygen species (ROS). PCL-NFs were fabricated by the electrospinning technique and are investigated as potential dressing material focused on the release of usnic acid (PCL-USNIC NFs), and its encapsulation efficiency and kinetic release were analyzed by high performance liquid chromatography (HPLC). This investigation was performed by analyzing the usnic acid concentration as a function of the distance from the mat center point. The kinetic release analysis is also developed with the usnea ghattensis extract (PCL-USNEA NFs), performing a metabolomic analysis of the released molecules as a function of time by nuclear magnetic resonance (NMR). Usnic acid was revealed as the most relevant compound together with other molecules, such as sucrose, mannitol, arabitol or glycerol that generate apositive matrix effect on the release of usnic acid. Finally, we analize the cytotoxicity and the neuroprotective effect of PCL-USNEA and PCL-USNIC NFs using a human neuroblastoma cell line model. Negligible toxicity was appreciated for both polymeric systems, showing high protective effects in presence of highly oxidative environment (e.g. in presence of H2O2).
PB Elsevier
YR 2024
FD 2024-10-28
LK https://hdl.handle.net/20.500.14352/125046
UL https://hdl.handle.net/20.500.14352/125046
LA eng
NO De La Cabeza Fernández M, Sánchez M, Lozano-Chamizo L, Cristina Abreu A, Anguís-Morillas A, Divakar PK, Marciello M, Filice M, Gonzalez-Rumayor V, Fernández I, Contreras-Cáceres R, Gómez-Serranillos P. Electrospun nanofibers for localized drug release of a neuroprotective natural extract of Usnea ghattensis. European Journal of Pharmaceutics and Biopharmaceutics 2024;205:114552. https://doi.org/10.1016/j.ejpb.2024.114552.
NO Comunidad de Madrid
NO Ministerio de ciencia e Innovación (España)
NO Instituto de Salud Carlos III (España)
NO Centro Nacional de Investigaciones Cardiovasculares Carlos III (España)
DS Docta Complutense
RD 19 oct 2025