RT Journal Article
T1 Time-dependent effects of leptin on food intake and locomotor activity in goldfish
A1 Vivas García, Yurena
A1 Azpeleta Noriega, Clara
A1 Feliciano, Andrea
A1 Velarde, Elena
A1 Isorna Alonso, Esther
A1 Delgado Saavedra, María Jesús
A1 Pedro Ormeño, Nuria De
AB The present study investigates the possible circadian dependence of leptin effects on food intake, locomotor activity, glycemia and plasma cortisol levels in goldfish (Carassius auratus). Fish were maintained under 12L:12D photoperiod and subjected to two different feeding schedules, one group fed during photophase (10:00) and the other one during scotophase (22:00). Leptin or saline were intraperitoneally injected at two different times (10:00 or 22:00), coincident or not with the meal time. To eliminate the entraining effect of the light/dark cycle, goldfish maintained under 24 h light (LL) were fed and leptin-injected at 10:00. A reduction in food intake and locomotor activity and an increase in glycemia were found in goldfish fed and leptin-injected at 10:00. No significant changes in circulating cortisol were observed. Those effects were not observed when leptin was administered during the scotophase, regardless the feeding schedule; neither in fish maintained under LL, suggesting that a day/night cycle would be necessary to observe the actions of leptin administered during the photophase. Changes in locomotor activity and glycemia were only observed in goldfish when leptin was injected at daytime, coincident with the feeding schedule, suggesting that these leptin actions could be dependent on the feeding time as zeitgeber. In view of these results it appears that the circadian dependence of leptin actions in goldfish can be determined by the combination of both zeitgebers, light/dark cycle and food. Our results point out the relevance of the administration time when investigating regulatory functions of hormones.
PB Elsevier
SN 0196-9781
YR 2011
FD 2011
LK https://hdl.handle.net/20.500.14352/116801
UL https://hdl.handle.net/20.500.14352/116801
LA eng
NO Vivas, Y., Azpeleta, C., Feliciano, A., Velarde, E., Isorna, E., Delgado, M. J., & De Pedro, N. (2011). Time-dependent effects of leptin on food intake and locomotor activity in goldfish. Peptides, 32(5), 989-995. https://doi.org/10.1016/j.peptides.2011.01.028
NO This work was supported by MEC (AGL2007-65744-C03-03/ACU) and BSCH-UCM (GR58/08) projects. C. Azpeleta and E. Velarde are predoctoral fellowship of MEC and MICINN, respectively.
NO Ministerio de Economía y Competitividad (España)
NO Universidad Complutense de Madrid
NO Banco Santander
DS Docta Complutense
RD 9 abr 2025