RT Journal Article
T1 Elevated intraocular pressure increases melatonin levels in the aqueous humour
A1 Alkozi, Hanan Awad
A1 Sánchez Naves, Juan
A1 Pérez de Lara, María Jesús
A1 Carracedo Rodríguez, Juan Gonzalo
A1 Fonseca Vázquez, Begoña
A1 Martínez Águila, Alejandro
A1 Pintor Just, Jesús Jerónimo
AB Purpose: To study the levels of melatonin in the aqueous humour of normotensive and hypertensive intraocular pressure (IOP) patients and to compare them to an animal model of glaucoma.Methods : A total of 37 eyes of 37 patients who underwent cataract surgery were included in the study and were divided into normotensive patients, with IOP below 21 mmHg (n = 23), and hypertensive patients, with IOP > 21 mmHg (n = 14). Glaucomatous DBA/2J (n = 6) and control C57BL/6J (n = 6) mice presenting 3 and 12 months of age for each strain were also used. Human and mice aqueous humours were aspirated using a 30-gauge Rycroft cannula on a tuberculin syringe and further processed to quantify melatonin by high-performance liquid chromatography analysis.Results: Melatonin levels in normotensive patients (IOP below 21 mmHg) presented values as medians (first quartile; third quartile) of 14.62 (5.38;37.99) ng/ml (n = 23), while hypertensive patients (IOP above 21 mmHg) showed melatonin concentrations of 46.63 (10.28; 167.28) ng/ml (n = 14; p < 0.039). Glaucoma mice presented melatonin values of 0.37 (0.34; 0.59) ng/ml (at 3 months of age, before the pathology starts), which increased to 1.55 (0.94; 1.88) ng/ml (at 12 months of age, when the pathology is fully developed and IOP is maximum; n = 6, p < 0.001). Control mice did not significantly modified melatonin concentrations between 3 and 12 months of age.Conclusion: Patients with high IOP present increased concentrations of melatonin in their aqueous humour compared to normotensive patients. This has been confirmed in a glaucomatous animal model in which it has been possible to see a correlation between the development of the pathology, with an increase in IOP, and a concomitant elevation of melatonin in the aqueous humour.
PB John Wiley & Sons Ltd.
SN 1755-375X
YR 2016
FD 2016-09-06
LK https://hdl.handle.net/20.500.14352/17666
UL https://hdl.handle.net/20.500.14352/17666
LA eng
NO Alkozi, H. A., Sánchez Naves, J., Pérez de Lara, M. J. et al. «Elevated Intraocular Pressure Increases Melatonin Levels in the Aqueous Humour». Acta Ophthalmologica, vol. 95, n.o 3, mayo de 2017. DOI.org (Crossref), https://doi.org/10.1111/aos.13253.
NO Redes Temáticas en Investigación Cooperativa en Salud
NO Universidad Complutense de Madrid
NO Gobierno de Arabia Saudí
DS Docta Complutense
RD 21 ene 2026