RT Journal Article
T1 The immunosuppressive effect of the tick protein, Salp15, is long-lasting and persists in a murine model of hematopoietic transplant
A1 Tomás-Cortázar, Julen
A1 Martín-Ruiz, Itziar
A1 Barriales, Diego
A1 Pascual-Itoiz, Miguel Ángel
A1 de Juan, Virginia Gutiérrez
A1 Caro-Maldonado, Alfredo
A1 Merino, Nekane
A1 Marina, Alberto
A1 Blanco, Francisco J.
A1 Flores Landeira, Juana María
A1 Sutherland, James D.
A1 Barrio, Rosa
A1 Rojas, Adriana
A1 Martínez-Chantar, María Luz
A1 Carracedo, Arkaitz
A1 Simó, Carolina
A1 García-Cañas, Virginia
A1 Abecia, Leticia
A1 Lavín, José Luis
A1 Aransay, Ana M.
A1 Rodríguez, Héctor
A1 Anguita, Juan
AB Salp15, a salivary protein of Ixodes ticks, inhibits the activation of naïve CD4 T cells. Treatment with Salp15 results in the inhibition of early signaling events and the production of the autocrine growth factor, interleukin-2. The fate of the CD4 T cells activated in the presence of Salp15 or its long-term effects are, however, unknown. We now show that Salp15 binding to CD4 is persistent and induces a long-lasting immunomodulatory effect. The activity of Salp15 results in sustained diminished cross-antigenic antibody production even after interruption of the treatment with the protein. Transcriptionally, the salivary protein provokes an acute effect that includes known activation markers, such as Il2 or Cd44, and that fades over time. The long-term effects exerted by Salp15 do not involve the induction of either anergy traits nor increased populations of regulatory T cells. Similarly, the treatment with Salp15 does not result in B cell anergy or the generation of myeloid suppressor cells. However, Salp15 induces the increased expression of the ectoenzyme, CD73, in regulatory T cells and increased production of adenosine. Our study provides a profound characterization of the immunomodulatory activity of Salp15 and suggests that its long-term effects are due to the specific regulation of CD73
PB Nature Research
YR 2017
FD 2017-09-06
LK https://hdl.handle.net/20.500.14352/125909
UL https://hdl.handle.net/20.500.14352/125909
LA eng
NO Tomás-Cortázar, J., Martín-Ruiz, I., Barriales, D., Pascual-Itoiz, M. Á., de Juan, V. G., Caro-Maldonado, A., Merino, N., Marina, A., Blanco, F. J., Flores, J. M., Sutherland, J. D., Barrio, R., Rojas, A., Martínez-Chantar, M. L., Carracedo, A., Simó, C., García-Cañas, V., Abecia, L., Lavín, J. L., Aransay, A. M., … Anguita, J. (2017). The immunosuppressive effect of the tick protein, Salp15, is long-lasting and persists in a murine model of hematopoietic transplant. Scientific reports, 7(1), 10740. https://doi.org/10.1038/s41598-017-11354-2
NO Author Contributions: Performed the experiments: J.T.C., I.M.M., D.B., M.A.P.I., V.G.J., A.C.M., N.M., A.M., J.M.F., C.S., V.G.C., J.A. Analyzed the data: J.T.C., J.L.L., A.M.A., H.R., J.A. Provided reagents: F.J.B., J.D.S., R.B., A.R., M.L.M.C. Wrote the manuscript: J.T.C., A.C., L.A., H.R., J.A., J.A. designed the study and provided supervision
NO Departamento de Educación del Gobierno Vasco
NO European Commission
NO Departamento Vasco de Industria, Turismo y Comercio
NO Departamento de Tecnología e Innovación de Bizkaia y la Red CIBERehd
NO Ministerio de Economía, Comercio y Empresa ( España )
DS Docta Complutense
RD 21 ene 2026