RT Journal Article
T1 A Metabolomics Study by 1H HRMAS NMR: From Sheep Milk to a Pressed-Curd Cheese: A Proof of Concept
A1 Castejón Ferrer, David
A1 Segura Plaza, José Francisco
A1 Cruz Díaz, Karen Paola
A1 Remiro Yagüe, Víctor
A1 Fernández Valle, María Encarnación
A1 Romero De Ávila Hidalgo, María Dolores
A1 Villa Valverde, Palmira
A1 Cambero Rodríguez, María Isabel
AB For the first time, High-Resolution Magic Angle Nuclear Magnetic Resonance spectroscopy (NMR-HRMAS) was applied to directly identify specific metabolites from a Spanish raw ewe’s milk and enzymatic coagulation pressed-curd cheese (Protected Geographical Indication: Castellano) manufactured by two procedures (traditional/artisanal vs. industrial) and including the ewe’s raw milk. The NMR parameters were optimized to study the complex matrixes of this type of cheese. In addition, conventional overcrowded 1H-NMR-HRMAS spectra were selectively simplified by a Carr–Purcell–Meiboom–Gill (CPMG) sequence or a stimulated echo pulse sequence by bipolar gradients (DIFF), thus modulating spin–spin relaxation times and diffusion of molecular components, respectively. 1H-NMR-HRMAS spectroscopy displayed important information about cheese metabolites, which can be associated with different manufacturing processes (industrial vs. traditional) and ripening times (from 2 to 90 days). These results support that this spectroscopy is a useful technique to monitor the ripening process, from raw milk to commercial ripened cheese, using a minimum intact sample, implying the absence of time-consuming sample pretreatments.
PB MDPI
YR 2024
FD 2024-04-13
LK https://hdl.handle.net/20.500.14352/105894
UL https://hdl.handle.net/20.500.14352/105894
LA eng
NO Castejón, D.; Segura, J.; Cruz-Díaz, K.P.; Remiro, V.; Fernández-Valle, M.E.; Romero de Ávila, M.D.; Villa, P.; Cambero, M.I. A Metabolomics Study by 1H HRMAS NMR: From Sheep Milk to a PressedCurd Cheese: A Proof of Concept. Analytica 2024, 5, 170–186. https:// doi.org/10.3390/analytica5020011
NO This article belongs to the Collection "Analytical and Applied Chemistry: the challenges and opportunities for growth in the 21st century"
NO Autorizado por la editorial: portada, índice y primeras páginas de la obra.
NO Ministerio de Ciencia, Innovación y Universidades (España)
DS Docta Complutense
RD 23 jul 2024