RT Journal Article
T1 Neutral molecular markers support common origin ofaluminium tolerance in three congeneric grass speciesgrowing in acidic soils
A1 Contreras, Roberto
A1 Figueiras, Ana M.
A1 Gallego Rodríguez, Francisco Javier
A1 Benavente, Elena
A1 Manzaneda, Antonio J.
A1 Benito Jiménez, César
AB Aluminium (Al) toxicity is the main abiotic stress limiting plant productivity in acidic soils that are widely distributed among arable lands. Plant species differ in the level of Al resistance showing intraspecific and interspecific variation in many crop species. However, the origin of Al-tolerance is not well known. Three annual species, difficult to distinguish phenotypically and that were until recently misinterpreted as a single complex species under Brachypodium distachyon, have been recently separated into three distinct species: the diploids B. distachyon (2n = 10) and B. stacei (2n = 20), and B. hybridum (2n = 30), the allotetraploid derived from the two diploid species. The aims of this work were to know the origin of Al-tolerance in acidic soil conditions within these three Brachypodium species and to develop new DNA markers for species discrimination. Two multiplex SSR-PCRs allowed to genotype a group of 94 accessions for 17 pentanucleotide microsatellite (SSRs) loci. The variability for 139 inter-microsatellite (ISSRs) markers was also examined. The genetic relationships obtained using those neutral molecular markers (SSRs and ISSRs) support that all Al-tolerant allotetraploid accessions of B. hybridum have a common origin that is related with both geographic location and acidic soils. The possibility that the adaptation to acidic soils caused the isolation of the tolerant B. hybridum populations from the others is discussed. We finally describe a new, easy, DNA barcoding method based in the upstream-intron 1 region of the ALMT1 gene, a tool that is 100 % effective to distinguish among these three Brachypodium species.
PB Annals of Botany Company
SN 2041-2851
YR 2017
FD 2017
LK https://hdl.handle.net/20.500.14352/18453
UL https://hdl.handle.net/20.500.14352/18453
LA eng
NO Ministerio de Educación y Ciencia (MEC)
NO Universidad Complutense de Madrid/Banco de Santander
DS Docta Complutense
RD 5 abr 2025