RT Journal Article
T1 Supercritical fluid extraction of oregano (Origanum vulgare) essentials oils: anti-inflammatory properties based on cytokine response on THP-1 macrophages
A1 Ocaña-Fuentes, Aurelio
A1 Señorans, Francisco Javier
A1 Reglero, Guillermo
A1 Arranz Gutiérrez, Elena María
AB Two fractions (S1 and S2) of an oregano (Origanum vulgare) extract obtained by supercritical fluid extraction have been used to test anti-inflammatory effects on activated human THP-1 cells. The main compounds present in the supercritical extract fractions of oregano were trans-sabinene hydrate, thymol and carvacrol. Fractions toxicity was assessed using the mitochondrial-respiration-dependent 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) reduction method for several concentrations during 24 and 48 h of incubation. Concentrations higher than 30 μg/mL of both supercritical S1 and S2 oregano fractions caused a reduction in cell viability in a dose-dependent manner. Oxidized-LDLs (oxLDLs) activated THP-1 macrophages were used as cellular model of atherogenesis and the release/secretion of cytokines (TNT-α, IL-1β, IL-6 and IL-10) and their respective mRNA expressions were quantified both in presence or absence of supercritical oregano extracts. The results showed a decrease in pro-inflammatory TNF-α, IL-1β and IL-6 cytokines synthesis, as well as an increase in the production of anti-inflammatory cytokine IL-10. These results may suggest an anti-inflammatory effect of oregano extracts and their compounds in a cellular model of atherosclerosis.
PB Elsevier
SN 0278-6915
YR 2010
FD 2010-03-21
LK https://hdl.handle.net/20.500.14352/91787
UL https://hdl.handle.net/20.500.14352/91787
LA eng
NO Ocana-Fuentes, A., Arranz-Gutierrez, E., Senorans, F. J., & Reglero, G. (2010). Supercritical fluid extraction of oregano (Origanum vulgare) essentials oils: anti-inflammatory properties based on cytokine response on THP-1 macrophages. Food and Chemical Toxicology, 48(6), 1568-1575.
NO Comunidad Autonoma de Madrid
NO Ministerio de Educación
NO Consolider Ingenio 2010
DS Docta Complutense
RD 24 mar 2026