RT Journal Article
T1 3D silicon doped hydroxyapatite scaffolds decorated with Elastin-like Recombinamers for bone regenerative medicine
A1 Vila, Mercedes
A1 García, Ana
A1 Girotti, Alessandra
A1 Alonso, Matilde
A1 Rodriguez Cabello, José Carlos
A1 González Vázquez, Arlyng
A1 Planell, Josep A.
A1 Engel, Elisabeth
A1 Buján, Julia
A1 Garcia-Honduvilla, Natalio
A1 Vallet Regí, María Dulce Nombre
AB The current study reports on the manufacturing by rapid prototyping technique of three-dimensional (3D) scaffolds based on silicon substituted hydroxyapatite with Elastin-like Recombinamers (ELRs) functionalized surfaces. Silicon doped hydroxyapatite (Si-HA), with Ca-10(PO4)(5.7)(SiO4)(0.3)(OH)(1.7)h(0.3) nominal formula, was surface functionalized with two different types of polymers designed by genetic engineering: ELR-RGD that contain cell attachment specific sequences and ELR-SNA15/RGD with both hydroxyapatite and cells domains that interact with the inorganic phase and with the cells, respectively. These hybrid materials were subjected to in vitro assays in order to clarify if the ELRs coating improved the well-known biocompatible and bone regeneration properties of calcium phosphates materials. The in vitro tests showed that there was a total and homogeneous colonization of the 3D scaffolds by Bone marrow Mesenchymal Stromal Cells (BMSCs). In addition, the BMSCs were viable and able to proliferate and differentiate into osteoblasts. Statement of Significance Bone tissue engineering is an area of increasing interest because its main applications are directly related to the rising life expectancy of the population, which promotes higher rates of several bone pathologies, so innovative strategies are needed for bone tissue regeneration therapies. Here we use the rapid prototyping technology to allow moulding ceramic 3D scaffolds and we use different bio-polymers for the functionalization of their surfaces in order to enhance the biological response. Combining the ceramic material (silicon doped hydroxyapatite, Si-HA) and the Elastin like Recombinamers (ELRs) polymers with the presence of the integrin-mediate adhesion domain alone or in combination with SNAI 5 peptide that possess high affinity for hydroxyapatite, provided an improved Bone marrow Mesenchymal Stromal Cells (BMSCs) differentiation into osteoblastic linkage. (C) 2016 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
PB Elsevier
SN 1742-7061
YR 2016
FD 2016
LK https://hdl.handle.net/20.500.14352/23100
UL https://hdl.handle.net/20.500.14352/23100
LA eng
NO RESEARCHER ID M-3378-2014 (María Vallet Regí)ORCID 0000-0002-6104-4889 (María Vallet Regí)
NO Unión Europea. FP7
NO Ministerio de Ciencia e Innovacion (MICINN)
NO Ministerio de Economía y Competitividad (MINECO)
NO CIBER-BBN
NO Agening Network of Excellence
NO EC
NO Junta de Castilla y León
NO LOREAL-Unesco Program for Women in Science
DS Docta Complutense
RD 27 mar 2026