RT Journal Article
T1 Double specific nested PCR and diagnostic SNP assay for species identification in lynx fecal critical samples
A1 Cruz, Alejandra
A1 Matallanas, Beatriz
A1 Lobón-Rovira, Javier
A1 Casanovas, Jorge G.
A1 Alonso Campos, Germán
A1 Arana Montes, María del Pilar
AB The single lynx species present in the Iberian Peninsula—Lynx pardinus (Temmink, 1827)—is one of the most threatened felines in the world so, any biological indication of its presence is significant. However, it is a very elusive species and physical evidence through direct visualization or camera trapping is difficult to obtain. Genetic noninvasive methods, in particular, molecular scatology, are a reliable alternative to unequivocally identify the presence of a species in a given geographical area. We have developed a highly specific and sensitive molecular method to identify the presence of this critically endangered lynx species from severely deteriorated fecal samples. The method consists in a double specific nested PCR of mitochondrialD-loop sequences and two diagnostic SNPs detected by a multiplex primer extension reaction.
PB Springer
SN 1877-7252
YR 2018
FD 2018-06-16
LK https://hdl.handle.net/20.500.14352/13180
UL https://hdl.handle.net/20.500.14352/13180
LA eng
NO Ministerio de Agricultura y Pesca, Alimentación y Medio Ambiente
DS Docta Complutense
RD 19 abr 2025