RT Journal Article
T1 Differences in virulence gene expression between atypical enteropathogenic Escherichia coli strains isolated from diarrheic and healthy ruminants
A1 Carrión Herrero, Francisco Javier
A1 Horcajo Iglesias, María Del Pilar
A1 Domínguez Bernal, Gustavo Ramón
A1 Fuente López, Ricardo De La
A1 Ruiz Santa Quiteria Serrano De La Cruz, José Antonio
A1 Orden Gutiérrez, José Antonio
AB Differences in the pathogenicity of atypical enteropathogenic Escherichia coli (EPEC) strains may be due, at least partially, to different expression patterns of some virulence genes. To investigate this hypothesis, the virulence gene expression patterns of 6 atypical EPEC strains isolated from healthy and diarrheic ruminants were compared using quantitative real-time reverse transcription polymerase chain reaction after growing the bacteria in culture medium alone or after binding it to HeLa epithelial cells. Some virulence genes in strains from diarrheic animals were upregulated relative to their expression in strains from healthy animals. When bacteria were cultured in the presence of HeLa cells, the ehxA and efa1/lifA genes, previously associated with the production of diarrhea, were expressed at higher levels in strains from diarrheic animals than in strains from healthy animals. Thus, the expression levels of some virulence genes may help determine which atypical EPEC strains cause diarrhea in ruminants.
PB Canadian Veterinary Medical Association
YR 2012
FD 2012
LK https://hdl.handle.net/20.500.14352/93859
UL https://hdl.handle.net/20.500.14352/93859
LA eng
NO Horcajo, P., Domínguez-Bernal, G., Carrión, J., De La Fuente, R., Ruiz-Santa-Quiteria, J. A., & Orden, J. A. (2013). Differences in virulence gene expression between atypical enteropathogenic Escherichia coli strains isolated from diarrheic and healthy ruminants. Canadian journal of veterinary research = Revue canadienne de recherche veterinaire, 77(2), 158–160
NO Universidad Complutense de Madrid
DS Docta Complutense
RD 13 abr 2025