RT Journal Article
T1 Osteoprotegerin in exosome-like vesicles from human cultured tubular cells and urine
A1 Benito-Martin, Alberto
A1 Zubiri, I
A1 Posada-Ayala, M
A1 Fernandez-Fernandez, Beatriz
A1 Cannata-Ortiz, P
A1 Sanchez-Nino, M.D
A1 Ruiz-Ortega, M
A1 Egido, J
A1 Ortiz, Alberto
A1 Ucero Herrería, Álvaro Conrado
A1 Álvarez Llamas, Gloria
AB Urinary exosomes have been proposed as potential diagnostic tools. TNF superfamily cytokines and receptors may be present in exosomes and are expressed by proximal tubular cells. We have now studied the expression of selected TNF superfamily proteins in exosome-like vesicles from cultured human proximal tubular cells and human urine and have identified additional proteins in these vesicles by LC-MS/MS proteomics. Human proximal tubular cells constitutively released exosome-like vesicles that did not contain the TNF superfamily cytokines TRAIL or TWEAK. However, exosome-like vesicles contained osteoprotegerin (OPG), a TNF receptor superfamily protein, as assessed by Western blot, ELISA or selected reaction monitoring by nLC-(QQQ)MS/MS. Twenty-one additional proteins were identified in tubular cell exosome-like vesicles, including one (vitamin D binding protein) that had not been previously reported in exosome-like vesicles. Twelve were extracellular matrix proteins, including the basement membrane proteins type IV collagen, nidogen-1, agrin and fibulin-1. Urine from chronic kidney disease patients contained a higher amount of exosomal protein and exosomal OPG than urine from healthy volunteers. Specifically OPG was increased in autosomal dominant polycystic kidney disease urinary exosome-like vesicles and expressed by cystic epithelium in vivo. In conclusion, OPG is present in exosome-like vesicles secreted by proximal tubular epithelial cells and isolated from Chronic Kidney Disease urine.
PB Public Library of Science
YR 2013
FD 2013-08-23
LK https://hdl.handle.net/20.500.14352/116691
UL https://hdl.handle.net/20.500.14352/116691
LA eng
NO 'Benito-Martin A, Ucero AC, Zubiri I, Posada-Ayala M, Fernandez-Fernandez B, Cannata-Ortiz P, Sanchez-Nino MD, Ruiz-Ortega M, Egido J, Alvarez-Llamas G, Ortiz A. Osteoprotegerin in exosome-like vesicles from human cultured tubular cells and urine. PLoS One. 2013 Aug 23;8(8):e72387'.
DS Docta Complutense
RD 16 abr 2025