RT Book, Section
T1 Intracellular calcium recording after purinoceptor activation using a video-microscopy equipment.
A1 Miras Portugal, María Teresa
A1 Ortega De La O, Felipe
A1 Gualix Sánchez, Francisco Javier
A1 Pérez Sen, Raquel
A1 García Delicado, Esmerilda
A1 Gómez Villafuertes, María Rosa
A2 Pelegrín, Pablo
AB Calcium is one of the most important intracellular messengers, triggering a wide range of cellular responses. Changes in intracellular free calcium concentration can be measured using calcium sensitive fluorescent dyes, which are either EGTA- or BAPTA-based organic molecules that change their spectral properties in response to Ca2+ binding. One of the most common calcium indicators is the ratiometric dye Fura-2. The main advantage of using ratiometric dyes is that the ratio signal is independent of the illumination intensity, dye concentration, photobleaching, and focus changes among others, allowing for the concentration of intracellular calcium to be determined independently of these artifacts. In this protocol, we describe the use of Fura-2 to measure intracellular calcium elevations in single cultured cells after purinoceptor activation using a video-microscopy equipment. This method, usually known as calcium imaging, allows for real-time quantification of intracellular calcium dynamics and can be adapted to measure agonist mediated intracellular calcium responses due to the activation of different purinergic receptors in several cellular models using the appropriate growth conditions.
PB Humana Press
SN 978-1-4939-9719-0
SN 978-1-4939-9716-9
SN 978-1-4939-9717-6
SN 1064-3745
YR 2020
FD 2020
LK https://hdl.handle.net/20.500.14352/130097
UL https://hdl.handle.net/20.500.14352/130097
LA eng
NO Miras-Portugal, M.T., Ortega, F., Gualix, J., Perez-Sen, R., Delicado, E.G., Gomez-Villafuertes, R. (2020). Intracellular Calcium Recording After Purinoceptor Activation Using a Video-Microscopy Equipment. In: Pelegrín, P. (eds) Purinergic Signaling. Methods in Molecular Biology, vol 2041. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9717-6_23
NO Red de Excelencia Consolider-Ingenio Spanish Ion Channel Initiative
NO Ministerio de Economía y Competitividad (España)
NO Comunidad de Madrid
NO Fundación Ramón Areces
DS Docta Complutense
RD 13 ene 2026