RT Journal Article
T1 An investigation of the utility of plasma Cytomegalovirus (CMV) microRNA detection to predict CMV DNAemia in allogeneic hematopoietic stem cell transplant recipients
A1 Talaya, Alberto
A1 Giménez, Estela
A1 Pascual, María Jesús
A1 Gago, Beatriz
A1 Piñana, José Luis
A1 Hernández Boluda, Juan Carlos
A1 Vázquez, Lourdes
A1 García, Magdalena
A1 Serrano, David
A1 Hernández Martín, Marta María
A1 Albert, Eliseo
A1 Solano, Carlos
A1 Navarro, David
AB Precise identification of patients at highest risk for developing Cytomegalovirus (CMV) DNAemia may improve CMV infection management in the allogeneic hematopoietic stem cell transplantation (allo-HSCT) setting. Here, we studied thepotential use of detecting free CMV micro(mi)RNAs circulating in plasma for predicting CMV DNAemia in this clinicalscenario. A total of 62 adult allo-HSCT recipients were included in this prospective observational multicenter study. PlasmaCMV DNA load was monitored using the CMV RealTime CMV PCR (Abbott Molecular, Des Plaines, IL, USA). Detection of mature CMV miRNAs in plasma drawn by days + 7, + 14 and + 30 after allo-HSCT was performed using the miScript PCR System (Qiagen, Hilden, Germany). Assays could be optimized for five out of the seven targeted CMV miRNAs: UL36-5p,US33-5p, UL148D, UL22A-5p and UL112-3p. Of the 62 patients included in the study, 42 developed a first episode of CMVDNAemia at a median of 35 days after allo-HSCT. All targeted CMV miRNA were detected early after transplantation, withCMV miRNA US33-5p and UL112-3p the most commonly found species at any time point; nevertheless, neither the detectionrate of CMV miRNAs nor their abundance allowed discrimination between patients with subsequent CMV DNAemia andthose with no CMV DNAemia. The data presented herein do not support any predictive utility of these CMV miRNAs forfirst episodes of CMV DNAemia in a cohort consisting primarily of allo-HSCT patients receiving haploidentical allografts.
PB SPRINGER
SN 0300-8584
SN 1432-1831
YR 2019
FD 2019-09-03
LK https://hdl.handle.net/20.500.14352/109957
UL https://hdl.handle.net/20.500.14352/109957
LA eng
NO Talaya, A., Giménez, E., Pascual, M. J., Gago, B., Piñana, J. L., Hernández-Boluda, J. C., Vázquez, L., García, M., Serrano, D., Hernández, M., Albert, E., Solano, C., & Navarro, D. (2020). An investigation of the utility of plasma Cytomegalovirus (CMV) microRNA detection to predict CMV DNAemia in allogeneic hematopoietic stem cell transplant recipients. Medical Microbiology and Immunology, 209(1), 15-21. https://doi.org/10.1007/S00430-019-00632-7
NO Fondo de Investigaciones Sanitarias (FIS)
DS Docta Complutense
RD 22 abr 2025