RT Journal Article T1 In vitro antifungal activity of topical and systemic antifungal drugs against Malassezia species A1 Carrillo‐Muñoz, Alfonso Javier A1 Rojas, Florencia A1 Tur‐Tur, Cristina A1 Sosa, María de los Ángeles A1 Ortiz Díez, Gustavo A1 Martín Espada, María Del Carmen A1 Paya Vicens, María Jesús A1 Giusiano, Gustavo AB The strict nutritional requirements of Malassezia species make it difficult to test the antifungal susceptibility. Treatments of the chronic and recurrent infections associated with Malassezia spp. are usually ineffective. The objective of this study was to obtain in vitro susceptibility profile of 76 clinical isolates of Malassezia species against 16 antifungal drugs used for topical or systemic treatment. Isolates were identified by restriction fragment length polymorphism. Minimal inhibitory concentrations (MIC) were obtained by a modified microdilution method based on the Clinical Laboratory Standards Institute reference document M27-A3. The modifications allowed a good growth of all tested species. High in vitro antifungal activity of most tested drugs was observed, especially triazole derivatives, except for fluconazole which presented the highest MICs and widest range of concentrations. Ketoconazole and itraconazole demonstrated a great activity. Higher MICs values were obtained with Malassezia furfur indicating a low susceptibility to most of the antifungal agents tested. Malassezia sympodialis and Malassezia pachydermatis were found to be more-susceptible species than M. furfur, Malassezia globosa, Malassezia slooffiae and Malassezia restricta. Topical substances were also active but provide higher MICs than the compounds for systemic use. The differences observed in the antifungals activity and interspecies variability demonstrated the importance to studying the susceptibility profile of each species to obtain reliable information for defining an effective treatment regimen.case">Malassezia restricta. Topical substances were also active but provide higher MICs than the compounds for systemic use. The differences observed in the antifungals activity and interspecies variability demonstrated the importance to studying the susceptibility profile of each species to obtain reliable information for defining an effective treatment regimen. PB Wiley SN 0933-7407 YR 2013 FD 2013-03-18 LK https://hdl.handle.net/20.500.14352/104658 UL https://hdl.handle.net/20.500.14352/104658 LA eng NO Carrillo-Muñoz, A.J., Rojas, F., Tur-Tur, C., de los Ángeles Sosa, M., Diez, G.O., Espada, C.M., Payá, M.J. and Giusiano, G. (2013), In vitro antifungal activity of topical and systemic antifungal drugs against Malassezia species. Mycoses, 56: 571-575. https://doi.org/10.1111/myc.12076 DS Docta Complutense RD 4 ago 2024