RT Journal Article
T1 Melatonin improves inflammation processes in liver of senescence-accelerated prone male mice (SAMP8)
A1 Cuesta Sancho, Sara
A1 Kireev, Roman
A1 Forman, Katherine
A1 García Martín, M. Cruz
A1 Escames, Germaine
A1 Ariznavarreta Ruiz, María Carmen
A1 Vara Ameigeiras, Elena María
A1 Fernández-Tresguerres Hernández, Jesús Ángel
AB Aging is associated with an increase in oxidative stress and inflammation. The aim of this study was to investigate the effect of aging on various physiological parameters related to inflammation in livers obtained from two types of male mice models: Senescence-accelerated prone (SAMP8) and senescence-accelerated-resistant (SAMR1) mice, and to study the influence of the administration of melatonin (1 mg/kg/day) for one month on old SAMP8 mice on these parameters.The parameters studied have been the mRNA expression of TNF-α, iNOS, IL-1β, HO-1, HO-2, MCP1, NFkB1, NFkB2, NFkB protein or NKAP and IL-10. All have been measured by real-time reverse transcription polymerase chain reaction RT-PCR. Furthermore we analyzed the protein expression of TNF-α, iNOS, IL-1β, HO-1, HO-2, and IL-10 by Western-blot.Aging increased oxidative stress and inflammation especially in the liver of SAMP8 mice. Treatment with melatonin decreased the mRNA expression of TNF-α, IL-1β, HO (HO-1 and HO-2), iNOS, MCP1, NFκB1, NFκB2 and NKAP in old male mice. The protein expression of TNF-α, IL-1β was also decreased and IL-10 increased with melatonin treatment and no significant differences were observed in the rest of parameters analyzed.The present study showed that aging was related to inflammation in livers obtained from old male senescence prone mice (SAMP8) and old male senescence resistant mice (SAMR1) being the alterations more evident in the former. Exogenous administration of melatonin was able to reduce inflammation.
PB Elsevier
SN 0531-5565
YR 2010
FD 2010-12
LK https://hdl.handle.net/20.500.14352/98339
UL https://hdl.handle.net/20.500.14352/98339
LA eng
NO Cuesta S, Kireev R, Forman K, García C, Escames G, Ariznavarreta C, Vara E, Tresguerres JA. Melatonin improves inflammation processes in liver of senescence-accelerated prone male mice (SAMP8). Exp Gerontol. 2010 Dec;45(12):950-6. doi: 10.1016/j.exger.2010.08.016
NO Instituto de Salud Carlos III
NO Junta de Andalucía
NO Instituto de Salud Carlos III
DS Docta Complutense
RD 9 abr 2025