RT Journal Article
T1 Magnetobiosensors Based on Viral Protein p19 for MicroRNA Determination in Cancer Cells and Tissues
A1 Campuzano Ruiz, Susana
A1 Torrente Rodríguez, Rebeca Magnolia
A1 López‐Hernández, Eva
A1 Conzuelo, Felipe
A1 Granados, Rosario
A1 Sánchez‐Puelles, José Maria
A1 Pingarrón Carrazón, José Manuel
AB MicroRNAs (miRs) have emerged as important clinical biomarkers with both diagnostic and prognostic value for relevant diseases, such as cancer. MiRs pose unique challenges for detection and are currently detected by northern blotting, real‐time PCR, and microarray techniques. These expensive, complicated, and time‐consuming techniques are not feasible for on‐site miR determination. In this study, amperometric magnetobiosensors involving RNA‐binding viral protein p19 as a selective biorecognition element were developed for miR quantification. The p19‐based magnetosensors were able to detect 0.4 fmol of a synthetic target and endogenous miR‐21 (selected as a model for its role in a wide variety of cancers) in only 2 h in total RNA extracted from cancer cells and human breast‐tumor specimens without PCR amplification and sample preprocessing. These results open up formidable perspectives for the diagnosis and prognosis of human cancers and for drug‐discovery programs.
PB Wiley
SN 1433-7851
YR 2014
FD 2014
LK https://hdl.handle.net/20.500.14352/96112
UL https://hdl.handle.net/20.500.14352/96112
LA eng
NO Campuzano S, Torrente-Rodríguez RM, López-Hernández E, Conzuelo F, Granados R, Sánchez-Puelles JM, Pingarrón JM. Magnetobiosensors based on viral protein p19 for microRNA determination in cancer cells and tissues. Angew Chem Int Ed Engl. 2014 Jun 10;53(24):6168-71. doi: 10.1002/anie.201403270. Epub 2014 Apr 30. PMID: 24789269.
NO  Ministerio de Economía y Competitividad (España)
NO Comunidad de Madrid
NO European Commission
NO Ministerio de Educación (España)
DS Docta Complutense
RD 23 abr 2025