RT Journal Article
T1 Transcriptomics of Canine Inflammatory Mammary Cancer Treated with Empty Cowpea Mosaic Virus Implicates Neutrophils in Anti-Tumor Immunity
A1 Barreno San Antolín, Lucía
A1 Sevane Fernández, Natalia
A1 Valdivia Lara, Edgar Guillermo
A1 Alonso Miguel, Daniel
A1 Suárez Redondo, María
A1 Alonso Díez, Ángela
A1 Fiering, Steven
A1 Beiss, Veronique
A1 Steinmetz, Nicole F.
A1 Pérez Alenza, María De Los Dolores
A1 Peña Fernández, Laura Luisa
AB Canine inflammatory mammary cancer (IMC) is a highly aggressive and lethal cancer in dogs serving as a valuable animal model for its human counterpart, inflammatory breast cancer (IBC), both lacking effective therapies. Intratumoral immunotherapy (IT-IT) with empty cowpea mosaic virus (eCPMV) nanoparticles has shown promising results, demonstrating a reduction in tumor size, longer survival rates, and improved quality of life. This study compares the transcriptomic profiles of tumor samples from female dogs with IMC receiving eCPMV IT-IT and medical therapy (MT) versus MT alone. Transcriptomic analyses, gene expression profiles, signaling pathways, and cell type profiling of immune cell populations in samples from four eCPMV-treated dogs with IMC and four dogs with IMC treated with MT were evaluated using NanoString Technologies using a canine immune-oncology panel. Comparative analyses revealed 34 differentially expressed genes between treated and untreated samples. Five genes (CXCL8, S100A9, CCL20, IL6, and PTGS2) involved in neutrophil recruitment and activation were upregulated in the treated samples, linked to the IL17-signaling pathway. Cell type profiling showed a significant increase in neutrophil populations in the tumor microenvironment after eCPMV treatment. These findings highlight the role of neutrophils in the anti-tumor response mediated by eCPMV IT-IT and suggest eCPMV as a novel therapeutic approach for IBC/IMC.
PB MDPI
SN 1661-6596
YR 2023
FD 2023-09-13
LK https://hdl.handle.net/20.500.14352/104432
UL https://hdl.handle.net/20.500.14352/104432
LA eng
NO Barreno, L.; Sevane, N.; Valdivia, G.; Alonso-Miguel, D.; Suarez-Redondo, M.; Alonso-Diez, A.; Fiering, S.; Beiss, V.; Steinmetz, N.F.; Perez-Alenza, M.D.; et al. Transcriptomics of Canine Inflammatory Mammary Cancer Treated with Empty Cowpea Mosaic Virus Implicates Neutrophils in Anti-Tumor Immunity. Int. J. Mol. Sci. 2023, 24, 14034. https://doi.org/ 10.3390/ijms241814034
NO 2023 Descuento MDPI
NO Ministerio de Ciencia, Innovación y Tecnología (España)
NO Universidad Complutense de Madrid
DS Docta Complutense
RD 13 jun 2025