RT Journal Article
T1 Biometric Alterations of Mouse Embryonic Eye Structures Due to Short-Term Folic Acid Deficiency
A1 Sijilmassi, Ouafa
A1 López Alonso, José Manuel
A1 Río Sevilla, Aurora Del
A1 Murillo González, Jorge Alfonso
A1 Barrio Asensio, María Del Carmen
AB Purpose: Folic acid (FA) is an essential nutrient for normal embryonic development. FA deficiency (FAD) in maternal diet increases the risk of several defects among the progeny, especially, neural tube defects. The eye begins its development from the neural tube; however, the relationship between FAD and ocular development in the offspring has been little explored and it isn’t known how the FAD affects the formation of the eye. Our objective was to analyze the effect of maternal FAD on mouse embryos ocular biometry.Methods: Female mice C57/BL/6J were distributed into three different groups, according to the assigned diet: control group fed a standard FA diet (2 mg FA/kg), FAD group for short term fed (0 mg FA/kg + 1% succinylsulfathiazole) from the day after mating until day 14.5 of gestation, and FAD group for long term fed the same FA-deficient diet for 6 weeks prior mating and continued with this diet during gestation. A total of 57 embryos (19 embryos of each dietary group) at 14.5 gestational days were evaluated. As indicators of changes in ocular biometry, we analyze two parameters: area and circularity of the lens and whole eye, and the area of the retina. The program used in the treatment and selection of the areas of interest was ImageJ. The statistical analysis was performed by IBM SPSS Statistics 19.Results: Regarding the measures of the area, FA-deficient lenses and eyes were smaller than that of controls. We have also observed increase in the size of the neural retina, spatially, in embryos from females fed FAD diet during long term. On the other hand, as regard to circularity measures, we have seen that eyes and lenses were more circular than control.Conclusion: Maternal FAD diet for a very short term generates morphological changes in ocular structures to the offspring.
PB Taylor & Francis
SN 0271-3683
YR 2018
FD 2018-11
LK https://hdl.handle.net/20.500.14352/13141
UL https://hdl.handle.net/20.500.14352/13141
LA eng
NO Sijilmassi, O., López Alonso, J. M., Rio Sevilla, A. et al. «Biometric Alterations of Mouse Embryonic Eye Structures Due to Short-Term Folic Acid Deficiency». Current Eye Research, vol. 44, n.o 4, abril de 2019, pp. 428-35. DOI.org (Crossref), https://doi.org/10.1080/02713683.2018.1545911
NO Ministerio de Economía, Comercio y Empresa (España)
NO Ministerio de Sanidad (España)
NO Universidad Complutense de Madrid/Banco de Santander
DS Docta Complutense
RD 7 abr 2025