RT Journal Article
T1 A dual-gene-deleted ASFV Lv17/WB/Rie1-ΔCD candidate administered orally to wild boar confers DIVA-compatible protection against virulent challenge
A1 Barasona García-Arévalo, José Ángel
A1 Kosowska, Aleksandra
A1 González-García, Gabriela
A1 Díaz De Frutos, Marta
A1 Franzoni, Giulia
A1 Nicolussi, Paola
A1 Porras, Nestor
A1 Sánchez-Segovia, Mónica
A1 De Antonio-Gómez, Daniel
A1 Rueda, Paloma
A1 Barroso Arévalo, Sandra
AB African swine fever (ASF) continues to expand worldwide. Recent detection in wild boar in Spain highlights the urgent need for effective control tools, with oral vaccination as a key priority. Following previous evaluation of the attenuated Lv17/WB/Rie1 strain, we assess an improved derivative, Lv17/WB/Rie1-ΔCD, lacking EP402R (CD2v) and EP153R, replaced by GFP to abrogate haemadsorption and enable Differentiating-Infected-from-Vaccinated-Animals (DIVA) diagnostics. Vaccinated animals received either a single high dose (10 TCID₅₀) or a prime and re-exposure regimen (10 TCID₅₀ plus a 10 TCID₅₀). Animals were challenged intramuscularly with the virulent Armenia07 genotype II strain. The ΔCD vaccine was well tolerated, inducing only transient low-grade fever. Prime-re-exposure vaccination induced earlier seroconversion (mean 12 ± 4 dpv) and sterilizing immunity in 5/6 animals in the high dose group. Overall protection reached 90%, while all unvaccinated controls died within 7 days. Quantitative PCR revealed >10³-fold reductions in viral genome copies in blood and tissues versus controls. DIVA ELISA reliably distinguished vaccine-induced antibodies from infection-derived responses. These findings identify Lv17/WB/Rie1-ΔCD as a safer oral ASFV vaccine candidate, addressing concerns raised with the parental Lv17/WB/Rie1 by increasing attenuation and supporting multi-gene deletion strategies. Further studies on safety, transmission, genetic stability, and environmental behaviour are required before large-scale field trials.
PB Taylor & Francis
SN 0165-2176
YR 2026
FD 2026
LK https://hdl.handle.net/20.500.14352/134642
UL https://hdl.handle.net/20.500.14352/134642
LA eng
NO Barasona, J. A., Kosowska, A., González-García, G., Díaz-Frutos, M., Franzoni, G., Nicolussi, P., … Barroso-Arévalo, S. (2026). A dual-gene-deleted ASFV Lv17/WB/Rie1-ΔCD candidate administered orally to wild boar confers DIVA-compatible protection against virulent challenge. Veterinary Quarterly, 46(1). https://doi.org/10.1080/01652176.2026.2649573
NO Justificación de autores:Jose A. Barasona: Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Visualization, Writing – original draft; Aleksandra Kosowska: Data curation, Formal analysis, Investigation, Methodology, Resources, Software, Validation, Visualization, Writing – original draft; Gabriela González-García: Data curation, Formal analysis, Investigation, Methodology, Validation, Visualization, Writing – review & editing; Marta Díaz-Frutos: Data curation, Formal analysis, Investigation, Methodology, Validation, Writing – review & editing; Giulia Franzoni: Data curation, Formal analysis, Investigation, Methodology, Resources, Supervision, Validation, Visualization, Writing – review & editing; Paola Nicolussi: Investigation, Resources, Supervision, Validation, Writing – review & editing; Nestor Porras: Data curation, Investigation, Methodology, Writing – review & editing; Mónica Sánchez-Segovia: Data curation, Formal analysis, Methodology, Software, Validation, Visualization, Writing – review & editing; Daniel De Antonio-Gómez: Data curation, Investigation, Methodology, Writing – review & editing; Paloma Rueda: Conceptualization, Funding acquisition, Investigation, Resources, Supervision, Writing – review & editing; Sandra Barroso-Arévalo: Conceptualization, Formal analysis, Investigation, Methodology, Supervision, Validation, Visualization, Writing – original draft.Contratos/Becas:José Ángel Barasona: contrato Ramón y Cajal RYC2022-038060-IMarta Sánchez Segovia: Beca Doctoral CT2/25
NO European Commission
NO Ministerio de Ciencia, Innovación y Universidades (España)
NO Comunidad de Madrid
DS Docta Complutense
RD 28 abr 2026