RT Journal Article
T1 Nanostructured gold electrodes promote neural maturation and network connectivity
A1 Dominguez-Bajo, Ana
A1 Rosa, Juliana M.
A1 González-Mayorga, Ankor
A1 Rodilla González, Beatriz Loreto
A1 Arché-Nuñez, Ana
A1 Benayas, Esther
A1 Ocón, Pilar
A1 Pérez García, Lucas
A1 Camarero, Julio
A1 Miranda, Rodolfo
A1 González, M. Teresa
A1 Aguilar, Juan
A1 Lopez-Dolado, Elisa
A1 Serrano, María C.
AB Progress in the clinical application of recording and stimulation devices for neural diseases is still limited, mainly because of suboptimal material engineering and unfavorable interactions with biological entities. Nanotechnology is providing upgraded designs of materials to better mimic the native extracellular environment and attain more intimate contacts with individual neurons, besides allowing for the miniaturization of the electrodes. However, little progress has been done to date on the understanding of the biological impact that such neural interfaces have on neural network maturation and functionality. In this work, we elucidate the effect of a gold (Au) highly ordered nanostructure on the morphological and functional interactions with neural cells and tissues. Alumina-templated Au nanostructured electrodes composed of parallel nanowires of 160 nm in diameter and 1.2 mu m in length (Au-NWs), with 320 nm of pitch, are designed and characterized. Equivalent non-structured Au electrodes (Au-Flat) are used for comparison. By using diverse techniques in in vitro cell cultures including live calcium imaging, we found that Au-NWs interfaced with primary neural cortical cells for up to 14 days allow neural networks growth and increase spontaneous activity and ability of neuronal synchronization, thus indicating that nanostructured features favor neuronal network. The enhancement in the number of glial cells found is hypothesized to be behind these beneficial functional effects. The in vivo effect of the implantation of these nanostructured electrodes and its potential relevance for future clinical applicability has been explored in an experimental model of rat spinal cord injury. Subacute responses to implanted Au-NWs show no overt reactive or toxic biological reactions besides those triggered by the injury itself. These results highlight the translational potential of Au-NWs electrodes for in vivo applications as neural interfaces in contact with central nervous tissues including the injured spinal cord.
SN 0142-9612
YR 2021
FD 2021-10-15
LK https://hdl.handle.net/20.500.14352/99427
UL https://hdl.handle.net/20.500.14352/99427
LA eng
NO Ana Domínguez-Bajo, Juliana M. Rosa, Ankor González-Mayorga, Beatriz L. Rodilla, Ana Arché-Núñez, Esther Benayas, Pilar Ocón, Lucas Pérez, Julio Camarero, Rodolfo Miranda, M. Teresa González, Juan Aguilar, Elisa López-Dolado, María C. Serrano, Nanostructured gold electrodes promote neural maturation and network connectivity, Biomaterials 279 (2021) 121186
NO Union Europea (Programa FP 7)
NO Marie Sklodowska-Curie grant
NO Ministerio de Ciencia e Innovación (España)
NO Project BiSURE
NO 'Severo Ochoa’ Programme for Centres of Excellence
NO Comunidad de Madrid
DS Docta Complutense
RD 21 ago 2024