RT Journal Article
T1 A novel approach to produce phage single domain antibody fragments for the detection of gluten in foods
A1 García García, Aina
A1 García Lacarra, Teresa
A1 Martín De Santos, María Del Rosario
A1 González Alonso, María Isabel
A1 Madrid García, Raquel
AB In this study, we demonstrated the feasibility of isolating recombinant phage-antibodies against gluten from a non-immunized library of human single-domain antibodies (dAbs). Phage display technology enabled the selection of affinity probes by successive rounds of biopanning against a biotinylated synthetic peptide comprising repetitive immunogenic gluten motifs. The analysis of a wide representation of heterologous plant species corroborated that two of the isolated clones were specific to wheat, barley and rye proteins. The phage antibody selected as the most appropriate clone for the detection of gluten in foods (dAb8E-phage) was further applied in an indirect ELISA to the analysis of 50 commercial food samples. Although the limit of detection achieved did not improve those of current immunoassays, the proposed methodology could provide promising new pathways for the generation of recombinant antibodies that allow a comprehensive determination of gluten in foods, whilst replacing the need for animal immunization.
PB Elsevier
SN 0308-8146
YR 2020
FD 2020
LK https://hdl.handle.net/20.500.14352/99144
UL https://hdl.handle.net/20.500.14352/99144
LA eng
NO García-García, A., Madrid, R., González, I., García, T., Martín, R. (2019). A novel approach to produce phage single domain antibody fragments for the detection of gluten in foods. Food Chemistry, 321, 126685. DOI: 10.1016/j.foodchem.2020.126685
NO Ministerio de Economía, Industria y Competitividad (España)
NO Comunidad de Madrid
DS Docta Complutense
RD 6 abr 2025