RT Journal Article
T1 Experimental test of a method for determining causal connectivities of species in reactions
A1 Sánchez Torralba, Antonio
A1 Yu, Kristine
A1 Shen, Peidong
A1 Oefner, Peter J.
A1 Ross, John
AB Theoretical analysis has shown the possibility of determining causal connectivities of reacting species and the reaction mechanism in complex chemical and biochemical reaction systems by applying pulse changes of concentrations of one or more species, of arbitrary magnitude, and measuring the temporal response of as many species as possible. This method, limited to measured and pulsed species, is given here an experimental test on a part of glycolysis including the sequence of reactions from glucose to fructose 1,6-biphosphate, followed by the bifurcation of that sequence into two branches, one ending in glycerol 3-phosphate, the other in glyceraldehyde 3-phosphate. Pulses of concentrations of one species at a time are applied to the open system in a non-equilibrium stationary state, and the temporal responses in concentrations of six metabolites are measured by capillary zone electrophoresis. From the results of these measurements and the use of the theory for their interpretation, we establish the causal connectivities of the metabolites and thus the reaction mechanism, including the bifurcation of one chain of reactions into two. In this test case of the pulse method, no prior knowledge was assumed of the biochemistry of this system. We conclude that the pulse method is relatively simple and effective in determining reaction mechanisms in complex systems, including reactants, products, intermediates, and catalysts and their effectors. The method is likely to be useful for substantially more complex systems.
PB National Academy of Sciences USA
SN 0027-8424
YR 2003
FD 2003
LK https://hdl.handle.net/20.500.14352/117542
UL https://hdl.handle.net/20.500.14352/117542
LA eng
NO Torralba AS et al., Experimental test of a method for determining causal connectivities of species in reactions, 2003, Proc. Natl. Acad. Sci. USA, 100, 1494-1498
NO National Science Foundation
NO National Institutes of Health
NO Ministerio de Educación, Cultura y Deporte
DS Docta Complutense
RD 5 abr 2025