RT Journal Article T1 Characterization of a Ligilactobacillus salivarius Strain Isolated from a Cheese Seal Which Was Last Used in 1936 A1 Alba Rubio, Claudio A1 Arroyo Rodríguez, Rebeca A1 Fernández Álvarez, Leonides A1 Narbad, Arjan A1 Rodríguez Gómez, Juan Miguel AB Cheesemaking played a pivotal role in the life of the Pyrenean villages where cheese was a most prized commodity and the subject of much local competition. In one of them (Sasa de Sobrepuerto), Mrs. Sebastiana Palacio decided in 1877 to label all the cheeses made in her household with a seal to differentiate them from those made by other local producers. The cheese seal was last used in 1936 and, since then, it has been kept under excellent storage conditions. Since well-preserved cheese seals are rare, and bacterial cells may survive desiccation for long periods, the objective of this work was to isolate and characterize any lactic acid bacteria that survived in the seal. Analysis of the milky crust material revealed the presence of sheep caseins. Culture-based analysis led to the isolation of a strain of Bacillus licheniformis and a strain of Ligilactobacillus salivarius (L. salivarius SP36). The latter was characterized in vitro for safety and dairy-related functional properties. Its genome encodes several genes involved in protein, peptide, and amino acid catabolism, and flavor. Overall, the phenotypic and genetic features of this strain support a high potential for being used as adjunct culture in cheesemaking. PB MDPI SN 2304-8158 YR 2024 FD 2024-06-25 LK https://hdl.handle.net/20.500.14352/107475 UL https://hdl.handle.net/20.500.14352/107475 LA eng NO Alba, C.; Arroyo, R.; Fernández, L.; Narbad, A.; Rodríguez, J.M. Characterization of a Ligilactobacillus salivarius Strain Isolated from a Cheese Seal Which Was Last Used in 1936. Foods 2024, 13, 2005. https://doi.org/10.3390/ foods13132005 NO This article belongs to the Special Issue Recent Advances in Lactic Acid Bacteria and Metabolite for Food Quality DS Docta Complutense RD 6 abr 2025