RT Journal Article
T1 A high titer antibody response against P22 protein immunocomplex is not correlated with protection in naturally tuberculosis-infected goats
A1 Ortega, J.
A1 Agulló Ros, I.
A1 Roy, A.
A1 Moreno, I.
A1 Gómez Buendía, Alberto
A1 Romero Martínez, Beatriz
A1 Ferreras Colino, E.
A1 Juan Ferré, Lucía De
A1 Domínguez, M.
A1 Domínguez Rodríguez, Lucas José
A1 Risalde, M.A.
A1 Bezos Garrido, Javier
AB Caprine livestock are significant reservoirs of the Mycobacterium tuberculosis complex (MTBC),contributing to tuberculosis (TB) transmission among animals and humans. The P22 proteinimmunocomplex (P22PI), derived from bovine tuberculin, shows immunostimulating capacityand is used for TB diagnosis. This study assessed the immunogenicity and protective efficacyof P22PI in two groups of goats: 24 naïve goats (12 immunised, 12 controls) from a TB-freeherd, and 24 infected goats (12 immunised, 12 controls), referred to as pre-infected animals,from a M. bovis-infected herd. Both were exposed for 5 months to M. bovis-naturally infectedgoats. Reactors to single and comparative intradermal tuberculin (SIT and SCIT, respectively)tests and interferon-gamma release assay (IGRA) significantly increased (p < 0.05) in bothgroups 5 months’ post-exposure, with no significant differences between immunised andcontrol animals. However, immunised animals exhibited a significantly higher (p < 0.05)antibody response against P22PI. Most naïve animals (83.3%) and all pre-infected animalsdeveloped TB-compatible lesions, with extensive necrosis in the lungs and associated lymphnodes, compared to 50% and 83.3% of control animals, respectively. These findings suggestthat while P22PI stimulates an intense antibody response under the conditions of the presentstudy, it does not confer protection against TB and may exacerbate disease severity.
PB Taylor & Francis
SN 0165-2176
YR 2024
FD 2024
LK https://hdl.handle.net/20.500.14352/113081
UL https://hdl.handle.net/20.500.14352/113081
LA eng
NO Ortega, J., Agullo Ros, I., Roy, A., Moreno, I., Gómez Buendía, A., Romero, B., Ferreras Colina, E., de Juan, L., Dominguez, M., Dominguez, L., Risalde, M.A. & Bezos, J. (2024). A high titer antibody response against P22 protein immunocomplex is not correlated with protection in naturally tuberculosis-infected goats. The veterinary quarterly, 44(1), 16–30. https://doi.org/10.1080/01652176.2024.2429851
NO Justificación de autores:Concept formulation: JO, IAR, LD, MAR and JB. Methodology: JO, IAR, AR, IM, AGB, BR, EFC, LJ, MAR and JB. Data analysis: JO, IAR, LD, MD, MAR and JB. Writing the original draft: JO, IAR, MAR and JB. Critical revisions: all the authors. Funding acquisition: MD, MAR and JB.Becas FPU:Ortega,J. FPU18/05197Agulló Ros, I. FPU19/03969
NO European Commission
NO Ministerio de Ciencia, Innovación y Universidades (España)
NO Universidad de Córdoba (España)
DS Docta Complutense
RD 18 abr 2025