RT Journal Article
T1 Multispectral Imaging Method for Rapid Identification and Analysis of Paraffin-Embedded Pathological Tissues
A1 Sijilmassi, Ouafa
A1 López Alonso, José Manuel
A1 Río Sevilla, Aurora Del
A1 Barrio Asensio, María Del Carmen
AB The study of the interaction between light and biological tissue is of great help in the identification of diseases as well as structural alterations in tissues. In the present study, we have developed a tissue diagnostic technique by using multispectral imaging in the visible spectrum combined with principal component analysis (PCA). We used information from the propagation of light through paraffin-embedded tissues to assess differences in the eye tissues of control mouse embryos compared to mouse embryos whose mothers were deprived of folic acid (FA), a crucial vitamin necessary for the growth and development of the fetus. After acquiring the endmembers from the multispectral images, spectral unmixing was used to identify the abundances of those endmembers in each pixel. For each acquired image, the final analysis was performed by performing a pixel-by-pixel and wavelength-by-wavelength absorbance calculation. Non-negative least squares (NNLS) were used in this research. The abundance maps obtained for the first endmember revealed vascular alterations (vitreous and choroid) in the embryos with maternal FA deficiency. However, the abundance maps obtained for the third endmember showed alterations in the texture of some tissues such as the lens and retina. Results indicated that multispectral imaging applied to paraffin-embedded tissues enhanced tissue visualization. Using this method, first, it can be seen tissue damage location and then decide what kind of biological techniques to apply.
PB Springer; Society for Imaging Informatics in Medicine
SN 1618-727X
YR 2023
FD 2023-04-18
LK https://hdl.handle.net/20.500.14352/73269
UL https://hdl.handle.net/20.500.14352/73269
LA eng
NO Ministerio de Sanidad (España)
NO Ministerio de Economía, Comercio y Empresa (España)
NO Universidad Complutense de Madrid
DS Docta Complutense
RD 23 jul 2024