RT Journal Article
T1 miR-203 imposes an intrinsic barrier during cellular reprogramming by targeting NFATC2
A1 Salazar Roa, María
A1 Martínez-Martínez, Sara
A1 Graña-Castro, Osvaldo
A1 Álvarez- Fernández, Mónica
A1 Trakala, Marianna
A1 Redondo, Juan-Miguel
A1 Malumbres, Marcos
AB Cellular reprogramming from somatic to pluripotent cells is the basis for multiple applications aimed to replace damaged tissues in regenerative medicine. However, this process is limited by intrinsic barriers that are induced in response to reprogramming factors. In this manuscript we report that miR-203, a microRNA with multiple functions in differentiation and tumor suppression, acts as an endogenous barrier to reprogramming. Genetic ablation of miR-203 results in enhanced reprogramming whereas its expression prevents the formation of pluripotent cells both in vitro and in vivo. Mechanistically, this effect correlates with the direct repression of NFATC2, a transcription factor involved in the early phases of reprogramming. Inhibition of NFATC2 mimics miR-203 effects whereas NFATC2 overexpression rescues inducible cell pluripotency in miR-203- overexpressing cultures. These data suggest that miR-203 repression may favor the efficiency of reprogramming in a variety of cellular models.
YR 2020
FD 2020
LK https://hdl.handle.net/20.500.14352/97408
UL https://hdl.handle.net/20.500.14352/97408
LA eng
DS Docta Complutense
RD 17 mar 2026