RT Journal Article
T1 Activation kinetics of regulatory molecules during immunological synapse in T cells
A1 Gómez Morón, Álvaro
A1 Requena, Silvia
A1 Roda Navarro, Pedro
A1 Martín Cófreces, Noa B.
AB T cell activation through TCR stimulation leads to the formation of the immunological synapse (IS), a specialized adhesion organized between T lymphocytes and antigen presenting cells (APCs) in which a dynamic interaction among signaling molecules, the cytoskeleton and intracellular organelles achieves proper antigen-mediated stimulation and effector function. The kinetics of molecular reactions at the IS is essential to determine the quality of the response to the antigen stimulation. Herein, we describe methods based on biochemistry, flow cytometry and imaging in live and fixed cells to study the activation state and dynamics of regulatory molecules at the IS in the Jurkat T cell line CH7C17 and primary human and mouse CD4+ T lymphocytes stimulated by antigen presented by Raji and HOM2 B cell lines and human and mouse dendritic cells.
PB Elsevier
SN 0065-1281
YR 2023
FD 2023-02-27
LK https://hdl.handle.net/20.500.14352/134091
UL https://hdl.handle.net/20.500.14352/134091
LA eng
NO Alvaro Gomez-Moron et al. Chapter 10 - Activation kinetics of regulatory molecules during immunological synapse in T cells. Methods in Cell Biology Volume 178, 2023, Pages 149-171
NO Agencia Estatal de Investigación (España)
DS Docta Complutense
RD 29 mar 2026