RT Journal Article
T1 Inhibitory Effect on In Vitro Streptococcus oralis Biofilm of a Soda-Lime Glass Containing Silver Nanoparticles Coating on Titanium Alloy
A1 Cabal, Belén
A1 Cafini, Fabio
A1 Esteban-Tejeda, Leticia
A1 Alou Cervera, Luis
A1 Bartolomé, José F.
A1 Sevillano Fernández, David
A1 López-Piriz, Roberto
A1 Torrecillas, Ramón
A1 Serafín Moya, José
A2 Wei-Chun Chin, 
AB This paper reports the effect of soda-lime-glass-nAg coating on the viability of an in vitro biofilm of Streptococcus oralis. Three strains (ATCC 35037 and two clinical isolates from periodontitis patients) were grown on coated with glass, glass containing silver nanoparticles, and uncoated titanium alloy disks. Two different methods were used to quantify biofilm formation abilities: crystal violet staining and determination of viable counts. The influence of the surface morphology on the cell attachment was studied. The surface morphology was characterized by scanning electron microscopy (SEM) and using a profilometer. SEM was also used to study the formation and the development of biofilm on the coated and uncoated disks. At least a >99.7% inocula reduction of biofilm respect to titanium disks and also to glass coated disks was observed in the glass-nAg coated disks for all the studied strains. A quantitative evaluation of the release of silver was conducted in vitro to test whether and to what extend the biocidal agent (silver) could leach from the coating. These findings suggest that the biofilm formation of S. oralis strains is highly inhibited by the glass-nAg and may be useful for materials which require durable antibacterial effect on their surfaces, as it is the case of dental implants.
PB PLOS
SN 1932-6203
YR 2012
FD 2012-08-03
LK https://hdl.handle.net/20.500.14352/107044
UL https://hdl.handle.net/20.500.14352/107044
LA eng
NO Cabal B, Cafini F, Esteban-Tejeda L, Alou L, Bartolomé JF, Sevillano D, López-Piriz R, Torrecillas R, Moya JS. Inhibitory effect on in vitro Streptococcus oralis biofilm of a soda-lime glass containing silver nanoparticles coating on titanium alloy. PLoS One. 2012;7(8):e42393.
NO Ministerio de Ciencia e Innovación (España)
DS Docta Complutense
RD 6 abr 2025