%0 Journal Article
%A Wu, Dan
%A Durán Sampedro, Gonzalo
%A Fitzgerald, Sheila
%A Garre, Massimiliano
%A O'Shea, Donal 
%T Double click macrocyclization with Sondheimer diyne of aza-dipyrrins for B–Free bioorthogonal imaging
%D 2023
%@ 1359-7345
%U https://hdl.handle.net/20.500.14352/94999
%X Sequential azide/diyne cycloadditions proved highly effective for the macrocyclization of a bis-azido aza-dipyrrin. Macrocyclic aza-dipyrrin could be produced in 30 min at rt in water with changes in fluorescence intensity and lifetimes measurable upon reaction. Live cell microscopy showed that aza-dipyrrins were suitable for confocal and STED super-resolution imaging and a bioorthogonal response to macrocyclization could be detected in cellular compartments. These results will encourage a broader examination of the sensing and imaging uses of aza-dipyrrins.
%~