RT Journal Article
T1 The effects of adiponectin and leptin on human endothelial cell proliferation: a live-cell study
A1 Álvarez, Granada
A1 Visitación Bartolomé, M.
A1 Miana, María
A1 Jurado López, Raquel
A1 Martín, Rubén
A1 Zuluaga Arias, María del Pilar
A1 Martínez Martínez, Ernesto
A1 Nieto, María Luisa
A1 Álvarez Sala, Luis A.
A1 Millán, Jesús
A1 Lahera Julia, Vicente
A1 Cachofeiro Ramos, María Victoria
AB The effect of adiponectin and leptin on the proliferation of the human microvascular endothelial cell line (HMEC-1) was studied in the absence or presence of fetal bovine serum (FBS). The participation of extracellular signal-regulated kinase (ERK) and phosphatidylinositol 3-kinase/Akt (PI-3K/Akt) pathways in this effect were evaluated. We studied the effect of both adipokines on the motility, mitosis, proliferation and cell death processes of HMEC-1 cells using live-cell imaging techniques. Adiponectin but not leptin further increased the proliferative effect induced by FBS on HMEC-1. This effect seems to be the consequence of an increase in the mitotic index in adiponectin-treated cells when compared to untreated ones. The presence of either the mitogen-activated protein kinase (MAPK) inhibitor (PD98059), or PI-3K inhibitor (LY294002), reduced the effect of adiponectin in a dose-dependent manner. Neither adipokine was able to affect HMEC-1 proliferation in FBS-free conditions. Duration of mitosis, cell motility and the cell death process were similar in all conditions. These data suggest that adiponectin and leptin exert different effects on endothelial cell function. Adiponectin was able to potentiate proliferation of HMEC-1. This effect involves the activation of both PI3-K/Akt and ERK/MAPK pathways. However, it seems to exert minimal effects on HMEC-1 function in the case of leptin
PB Karger
SN 1018-1172
YR 2012
FD 2012
LK https://hdl.handle.net/20.500.14352/44609
UL https://hdl.handle.net/20.500.14352/44609
LA eng
NO Álvarez, G., Visitarión Bartolomé, M., Miana, M. et al. «The Effects of Adiponectin and Leptin on Human Endothelial Cell Proliferation: A Live-Cell Study». Journal of Vascular Research, vol. 49, n.o 2, 2012, pp. 111-22. DOI.org (Crossref), https://doi.org/10.1159/000332332.
NO Fondo de Investigaciones Sanitarias
NO Mutua Madrileña
NO Red Cardiovascular del FIS
DS Docta Complutense
RD 12 may 2025