RT Journal Article
T1 Proteomic characterization of EPCs and CECs “in vivo” from acute coronary syndrome patients and control subjects
A1 Mourino-Alvarez, L.
A1 Calvo, E.
A1 Moreu, J.
A1 Padial, L.R.
A1 López, J.A.
A1 Barderas, M.G.
A1 Gil Dones, Félix
AB Background:Circulating endothelial cells (CECs) and endothelial progenitor cells (EPCs) represent two scarce blood populations that are thought to play important roles in tissue vascularization. They have also been proposed as potential markers for more than a dozen pathologies. Moreover, EPCs have arisen as a new therapeutic option for cardiovascular disease. However nowadays there is certain controversy about their roles and a better understanding of EPC biology is required to develop new strategies for forthcoming therapies.Methods:Flow cytometry analysis was performed on freshly isolated mononuclear cells from control subjects and Acute Coronary Syndrome (ACS) patients. EPCs and CECs for both groups were isolated and quantified. Statistical analyses were performed to test the potential biomarker usefulness of both populations in ACS together with the first “in vivo” proteomic characterizations of these populations.Results:Our results do not show statistical differences in the quantification of CECs and EPCs in control subjects and ACS patients. The proteomic characterization allowed us to identify 673 proteins associated to CECs (389 in controls and 462 in ACS patients), and another 502 proteins in EPCs (350 in controls and 274 in ACS patients).
PB ScienceDirect
SN 0304-4165
YR 2013
FD 2013-04
LK https://hdl.handle.net/20.500.14352/94591
UL https://hdl.handle.net/20.500.14352/94591
LA eng
NO Fondo de Investigación Sanitaria de Castilla la Mancha
DS Docta Complutense
RD 31 ago 2024