RT Book, Section
T1 Cell Population Analyses of Neurospheres Generated In Vitro  from Ovarian Cortical Cells in Mammals: Characterization  by Immunohistochemistry and Flow Cytometry.
A1 González Gil, Alfredo
A1 Sánchez Maldonado, María Belén
A1 Sánchez Pérez, María de los Ángeles
A1 Aranda Espinosam, Pedro José
A1 Picazo González, Rosa Ana
A2 Ortega De La O, Felipe
AB Alternative experimental models based upon isolation and culture of extra-neural NSC/NPC or adult stem cells that can be induced in vitro to generate these cells and NS rely on development of procedures that can demonstrate the identity of cells as NSC/NPC and their compromise with the neural lineage during culture. Gene expression analyses by RT-PCRQ, immunohistochemical localization of characteristic NSC/NPC antigens, analyses of cell populations by flow cytometry, or ultrastructural analyses of in vitro generated NS are the most frequent and reliable methods to demonstrate the molecular and structural hallmarks of NSC/NPCs and NS. Among all these techniques, those aimed to immunolocalize specific NSC/NPC antigens on a cell basis and in a time-dependent fashion throughout culture require skillful NS processing before setting up the technique. In this chapter, comprehensive protocols for grouped OCC-NS preaggregation and paraffin embedding for immunolocalization of NSC/NPC antigens and NS population analyses by flow cytometry are fully explained.
PB Humana Press
SN 978-1-0716-4385-3
SN 978-1-0716-4388-4
SN 978-1-0716-4386-0
SN 1064-3745
YR 2025
FD 2025
LK https://hdl.handle.net/20.500.14352/130811
UL https://hdl.handle.net/20.500.14352/130811
LA eng
NO Alfredo González-Gil, Belén Sánchez-Maldonado, Ángeles Sánchez Pérez, Pedro Jose´Aranda, and Rosa Ana Picazo (2025) Cell Population Analyses of Neurospheres Generated In Vitro  from Ovarian Cortical Cells in Mammals: Characterization  by Immunohistochemistry and Flow Cytometry . In Methods in Molecular Biology, Neural Stem Cells. Methods ans Protocols, Chapter 20, pp 289-309. Editor Felipe Ortega De La O. Ed. Springuer Nature, New York, USA.
NO Ministerio de Economía y Competitividad (España)
NO Universidad Complutense de Madrid
NO Banco Santander
DS Docta Complutense
RD 18 mar 2026