RT Journal Article
T1 Combination of trained immunity and specific inactivated vaccine against S. Choleraesuis infection in pigs
A1 Sánchez Morales, Lidia
A1 Pérez Sancho, Marta
A1 García-Seco Romero, María Teresa
A1 Balseiro, Ana
A1 Pérez-Domingo, Andrea
A1 Buendía, Aránzazu
A1 Díez Guerrier, Alberto Antoine
A1 Fernández Manzano, Álvaro
A1 Relimpio, David
A1 Ugarte Ruiz, María
A1 Risalde, María de los Ángeles
A1 Gortázar, Christian
A1 Domínguez Rodríguez, Lucas José
AB Introduction: Salmonella enterica subsp. enterica serovar Choleraesuis is a pathogen that can cause severe systemic disease and remains a challenge in swine production due to its impact on animal health, economic losses, and zoonotic risk. While vaccination is a key control tool, current vaccines often provide limited protection to the targeted (and untargeted) serovar. Trained immunity has gained attention as a possible way to enhance non-specific protection.Methods: This study explored whether a porcine-derived BCG strain (dpB), administered live or inactivated, could improve the protective effect of a conventional S. Choleraesuis vaccine in pigs, not only against homologous challenge but also against heterologous serovars.Results: Pigs immunized with inactivated dpB in conjunction with the S. Choleraesuis vaccine showed a trend toward better bacterial control, with the lowest levels of S. Choleraesuis recovered from feces and ileocecal lymph nodes. Additionally, this group demonstrated a slight improved immune reactivity to heterologous serovars and a balanced cytokine response, without indications of excessive inflammation. In contrast, animals that received live dpB in addition to the vaccine showed the most severe pulmonary lesions, lowest phagocytic activity and a dysregulated cytokine profile.Discussion: These results suggest that inactivated dpB may modulate immune responses when administered together with a conventional S. Choleraesuis vaccine and could contribute to improved bacterial control. However, the observed effects were generally modest and require confirmation in larger studies. These preliminary results support further investigation of trained immunity-based approaches as potential strategies added to conventional vaccination against Salmonella in swine.
PB Frontiers Media
YR 2026
FD 2026
LK https://hdl.handle.net/20.500.14352/138948
UL https://hdl.handle.net/20.500.14352/138948
LA eng
NO Sánchez-Morales L, Pérez-Sancho M, García-Seco T, Balseiro A, Pérez-Domingo A, Buendía A, Díez-Guerrier A, Fernández-Manzano Á, Relimpio D, Ugarte-Ruiz M, de los Ángeles Risalde M, Gortázar C and Domínguez L (2026) Combination of trained immunity and specific inactivated vaccine against S. Choleraesuis infection in pigs. Front. Vet. Sci. 13:1879508. doi: 10.3389/fvets.2026.1879508
NO Justificación de autores:LS-M: Formal analysis, Investigation, Methodology, Writing – original draft, Writing – review & editing. MP-S: Conceptualization, Project administration, Supervision, Writing – review & editing. TG-S: Data curation, Formal analysis, Investigation, Writing – review & editing. ABa: Methodology, Writing – review & editing. AP-D: Methodology, Writing – review & editing. ABu: Methodology, Writing – review & editing. AD-G: Methodology, Writing – review & editing. ÁF-M: Methodology, Writing – review & editing. DR: Methodology, Writing – review & editing. MU-R: Methodology, Writing – review & editing. MÁ: Methodology, Writing – review & editing. CG: Conceptualization, Methodology, Writing – review & editing. LD: Conceptualization, Funding acquisition, Project administration, Supervision, Writing – review & editing.
DS Docta Complutense
RD 20 ago 2026