RT Journal Article
T1 High hydrostatic pressure assisted by food-grade enzymes as a sustainable approach for the development of an antioxidant ingredient
A1 Peña Armada, Rocío de la
A1 Rupérez, Pilar
A1 Villanueva Suárez, María José
A1 Mateos-Aparicio Cediel, Inmaculada
AB Apple is one of the most important fruits in the world. The elaboration of apple-based products such as cider or apple juice generates around 3–4.2 million tonnes of apple by-product that are annually discarded. Apple byproduct has been described as an interesting source of bioactive compounds, including polyphenols. These valuable components, once extracted, could enable the usage of the apple by-product as a natural antioxidant ingredient. The aim of the present work was to address the effect of high hydrostatic pressure (HHP), and HHP assisted by two alternative food-grade enzymes, Celluclast®, and Ultraflo®. For that purpose, the total phenolic content was measured by Folin-Ciocalteu and Fast-Blue BB assays. Furthermore, antioxidant capacity was determined by three analytical methods (DPPH, ORAC and FRAP) and the characterization of phenolic compounds was carried out by Chromatography and Mass Spectrometry analysis. Results indicated a significant increase of the total phenolic content and the antioxidant capacity after the HHP aided by Celluclast® or Ultraflo® treatment. Conversely, the exclusive application of HHP induced minor changes. Thus, the combination of HHP and the food-grade enzymes Celluclast® or Ultraflo®, has proved to be an effective strategy for the extraction of natural antioxidant compounds from the apple by-product
PB Elsevier
SN 0023-6438
YR 2022
FD 2022-09-18
LK https://hdl.handle.net/20.500.14352/72113
UL https://hdl.handle.net/20.500.14352/72113
LA eng
NO De La Peña Armada, R., Rupérez, P., Villanueva Suárez, M. J. et al. «High Hydrostatic Pressure Assisted by Food-Grade Enzymes as a Sustainable Approach for the Development of an Antioxidant Ingredient». LWT, vol. 169, noviembre de 2022, p. 113968. DOI.org (Crossref), https://doi.org/10.1016/j.lwt.2022.113968.
NO CRUE-CSIC (Acuerdos Transformativos 2022)
NO Ministerio de Economía, Comercio y Empresa (España)/Fondo Europeo de Desarrollo Regional
DS Docta Complutense
RD 10 abr 2025